Lefty2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:116645 Cota CD, et al., Mice with mutations in Mahogunin ring finger-1 (Mgrn1) exhibit abnormal patterning of the left-right axis. Dev Dyn. 2006 Dec;235(12):3438-47
Assay type:	RNA in situ
MGI Accession ID:	MGI:5553303
Gene symbol:	Lefty2
Gene name:	left-right determination factor 2

Probe:	Lefty probe
Probe preparation:	Antisense
Assay notes:	Probe used recognizes both family members.

Results

5D ©

Specimen 5D: embryonic day 8.0-8.5 (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	5D	Expressed in the left side.

5E ©

5J ©

Specimen 5E/J: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	5E , 5J	50% mutants had normal expression in the left side.

5F ©

Specimen 5F: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	5F	50% mutants had normal expression in the left side.

5G ©

Specimen 5G: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Widespread	5G	25% mutants expressed bilaterally.

5H ©

Specimen 5H: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	5H	3% mutants had strong expression in the right side. Low expression in the left side.

5I ©

Specimen 5I: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Uniform	5I	25% mutants had symmetric expression in the right and left side.

5K ©

Specimen 5K: embryonic day 8.0-8.5; Mgrn1^md-2J/Mgrn1^md-2J (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	5K	50% mutants had normal expression in the left side.

5L ©

Specimen 5L: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 2-3 somites

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Uniform	5L	25% mutants expressed bilaterally.

5M ©

Specimen 5M: embryonic day 8.0; Mgrn1^md-2J/Mgrn1^md-2J (more

)
Note: 2-3 somites

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Uniform	5M	25% mutants expressed bilaterally.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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