Gene Expression Data
Assay Details
Assay
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Reference:
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J:169913
Hudson LD, et al., A tool for examining the role of the zinc finger myelin transcription factor 1 (Myt1) in neural development: Myt1 knock-in mice. Transgenic Res. 2011 Aug;20(4):951-61
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5553340
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Gene symbol:
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Myt1
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Gene name:
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myelin transcription factor 1
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Reporter:
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GFP
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Antibody:
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anti-GFP (Invitrogen)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The fixative included picric acid.
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Results
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Specimen
4A/C +/-:
(close )
Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/Myt1+
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Widespread |
4C +/-
,
4A +/-
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Labeled cells are observed throughout the spinal cord. Similar numbers of labeled cells are seen in heterozygotic and homozygotic animals. Cells do not double-label with a neural marker, indicating these are undifferentiated neural progenitors.
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Specimen
4A/C -/-:
(close )
Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/Myt1tm1(EGFP/icre)Ldh
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Widespread |
4A -/-
,
4C -/-
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Labeled cells are observed throughout the spinal cord. Similar numbers of labeled cells are seen in heterozygotic and homozygotic animals. Cells do not double-label with a neural marker, indicating these are undifferentiated neural progenitors.
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Specimen
5:
(close )
Genetic Background:
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B6.129S-Myt1tm1(EGFP/icre)Ldh
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Mutant Allele(s):
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Myt1tm1(EGFP/icre)Ldh/0
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
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5
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Labeled cells are observed throughout the spinal cord. These cells are not double-labeled with a marker for mature neurons.
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