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Gene Expression Data
Assay Details
Assay
Reference: J:132132 Mahtab EA, et al., Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev Dyn. 2008 Mar;237(3):847-57
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5559316
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 2a,b: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: proepicardium Present 2a , 2b

Specimen 2c,d: embryonic day 9.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS15: proepicardium Present 2c , 2d There were no marked differences in expression between the wild type and knockout.

Specimen 3b,d,e: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS18: pericardium Present 3d , 3e , 3b
TS18: visceral pericardium Present 3b , 3d , 3e Expression was detected in the epicardium.
TS18: mesothelium Present Regionally restricted 3b , 3e , 3d Expression was detected in coelomic mesothelium.

Specimen 3f,i,j: embryonic day 10.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS18: visceral pericardium Present 3f , 3j , 3i Expression in individual epicardium cells is unchanged in the mutant. However, there are less epicardium cells in the knockout mouse.
TS18: mesothelium Present Regionally restricted 3f , 3j , 3i Expression was detected in coelomic mesothelium.

Specimen 4h: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: visceral pericardium Present 4h Expression was detected in the epicardium.

Specimen 4i: embryonic day 12.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS20: visceral pericardium Present 4i Expression was detected in the epicardium, but there was dissociation of the epicardium.

Specimen 4j: embryonic day 12.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS20: visceral pericardium Present 4j Expression was detected in the epicardium, but there was dissociation of the epicardium.

Specimen 5e: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: visceral pericardium Present 5e Expression was detected in the epicardium.

Specimen 5f: embryonic day 14.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS22: visceral pericardium Present Patchy 5f Expression was detected in the epicardium.

Specimen not shown: embryonic day 15.5; Pdpntm1Acgg/Pdpntm1Acgg (more )
Structure Level Pattern Image Note
TS23: visceral pericardium Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory