Antibody:	anti-E-cadherin (H-108; sc-7870)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: proepicardium	Present		2i , 2f , 2e
TS15: sinus venosus	Present	Regionally restricted	2f , 2e , 2i	Expression was detected in the mesenchyme of the sinus venosus region.

Structure	Level	Pattern	Image	Note
TS15: proepicardium	Present		2k , 2h , 2g	Expression was up-regulated in the knockout proepicardial organ compared with wild type.
TS15: sinus venosus	Present	Regionally restricted	2h , 2g , 2k	Expression was up-regulated in the mesenchyme of the sinus venosus region in the knockout compared to the wild type.

Structure	Level	Pattern	Image	Note
TS18: visceral pericardium	Present		3k , 3g	Expression was detected in the epicardium.
TS18: mesothelium	Present	Regionally restricted	3k , 3g	Expression was detected in coelomic mesothelium.

Structure	Level	Pattern	Image	Note
TS18: visceral pericardium	Present		3h , 3l	Expression was more extensive in the epicardium of the knockout compared with the wild type.
TS18: mesothelium	Present	Regionally restricted	3h , 3l	Expression was detected in coelomic mesothelium.