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Gene Expression Data
Assay Details
Assay
Reference: J:142645 Sherwood RI, et al., Transcriptional dynamics of endodermal organ formation. Dev Dyn. 2008 Dec 18;238(1):29-42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5562658
Gene symbol: Foxa2
Gene name: forkhead box A2
Antibody: anti-Foxa2 (M-20; sc-6554)
Detection system: Secondary antibody
Assay notes: Confocal microscopy.
Results
Specimen 1A,B: embryonic day 9.5 (more )
Note: Boxed region in A is magnified in B.
Structure Level Pattern Image Note
TS15: embryo endoderm Present 1A , 1B
TS15: floor plate Present 1A , 1B
TS15: neural tube floor plate Present 1A , 1B
TS15: notochord Present 1A , 1B

Specimen 1D: embryonic day 9.5 (more )
Note: Double labeled: red - Foxa2; green - Epcam (assay MGI:5562885).
Structure Level Pattern Image Note
TS15: embryo endoderm Present 1D
TS15: floor plate Present 1D
TS15: neural tube floor plate Present 1D
TS15: notochord Present 1D

Specimen 3A: embryonic day 9.5 (more )
Note: Boxed region is magnified in right panel. Double labeled: red - Foxa2; green - Cdx2 (assay MGI:5562893).
Structure Level Pattern Image Note
TS15: embryo endoderm Present 3A left panel , 3A right panel
TS15: gut Present 3A left panel , 3A right panel

Specimen 3E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: embryo endoderm Present Regionally restricted 3E Expression is in the nascent stomach, pancreas, liver, and intestinal endoderm.
TS15: gut Present Regionally restricted 3E Expression is in the nascent stomach, pancreas, liver, and intestinal endoderm.
TS15: pancreas primordium Present Regionally restricted 3E Expression is in the nascent stomach, pancreas, liver, and intestinal endoderm.

Specimen 4A: embryonic day 8.25 (more )
Note: 4-6 somites. Triple labeled: blue - Foxa2; green - Sox2 (assay MGI:5562906); red - Cdx2 (assay MGI:5562893).
Structure Level Pattern Image Note
TS13: gut Present Regionally restricted 4A right panel

Specimen 6A: embryonic day 8.25 (more )
Note: 4-6 somites. Double labeled: red - Foxa2; green - Mnx1 (assay MGI:5562904).
Structure Level Pattern Image Note
TS13: gut endoderm Present 6A right panel

Specimen 6B: embryonic day 8.5 (more )
Note: 9-11 somites. Double labeled: red - Foxa2; green - Mnx1 (assay MGI:5562904).
Structure Level Pattern Image Note
TS13: gut endoderm Present 6B right panel

Specimen 6C: embryonic day 9.5 (more )
Note: Double labeled: red - Foxa2; green - Mnx1 (assay MGI:5562904).
Structure Level Pattern Image Note
TS15: embryo endoderm Present 6C right panel
TS15: gut Present 6C right panel


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory