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Gene Expression Data
Assay Details
Assay
Reference: J:142645 Sherwood RI, et al., Transcriptional dynamics of endodermal organ formation. Dev Dyn. 2008 Dec 18;238(1):29-42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5562904
Gene symbol: Mnx1
Gene name: motor neuron and pancreas homeobox 1
Antibody: anti-Hlxb9
Detection system: Secondary antibody
Assay notes: Confocal microscopy.
Results
Specimen 3B: embryonic day 9.5 (more )
Note: Triple labeled: green - Mnx1; blue - Pdx1 (assay MGI:5562896); red - Prox1 (assay MGI:5562898).
Structure Level Pattern Image Note
TS15: pancreas primordium Present 3B Hb9/merge Expression is in the dorsal pancreatic region endoderm.
Specimen 6A: embryonic day 8.25 (more )
Note: 4-6 somites. Double labeled: green - Mnx1; red - Foxa2 (assay MGI:5562658).
Structure Level Pattern Image Note
TS13: gut endoderm Present Regionally restricted 6A left panel , 6A right panel Expression is in the medial-most half of the endoderm surrounding the notochord.
TS13: notochord Strong 6A right panel , 6A left panel
Specimen 6B: embryonic day 8.5 (more )
Note: 9-11 somites. Double labeled: green - Mnx1; red - Foxa2 (assay MGI:5562658).
Structure Level Pattern Image Note
TS13: gut endoderm Present Regionally restricted 6B right panel , 6B left panel Expression is in the 10-15 dorsal-most cell layers.
Specimen 6C: embryonic day 9.5 (more )
Note: Double labeled: green - Mnx1; red - Foxa2 (assay MGI:5562658).
Structure Level Pattern Image Note
TS15: embryo endoderm Strong Graded 6C left panel , 6C right panel Expression is strongest in the most dorsal endoderm and progressively weaker in more ventral cells.
TS15: gut Strong Graded 6C left panel , 6C right panel Expression is strongest in the most dorsal endoderm and progressively weaker in more ventral cells.
Specimen not shown: embryonic day 8.0 (more )
Structure Level Pattern Image Note
TS12: gut endoderm Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory