Probe:	Neurog2 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Scattered	1B	Expressed in a salt-and-pepper pattern in the dorsal telencephalon.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2E	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall ventricular layer	Present	Regionally restricted	2F	Expressed in the thalamus and eminentia thalami.
TS19: diencephalon lateral wall mantle layer	Present	Regionally restricted	2F	Expressed in the thalamus, and a part of the hypothalamus.
TS19: telencephalon	Present	Regionally restricted	2F	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2G	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall.
TS19: diencephalon	Present	Regionally restricted	2G	Expressed in the caudal diencephalon, including the thalamus, except in the prethalamus. No expression in the dorsocaudal part of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2H	Expressed in the rostral pretectum.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		3E
TS21: cerebral cortex ventricular layer	Strong		3E

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		3F
TS21: cerebral cortex ventricular layer	Strong		3F

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		3H , 3G
TS21: cerebral cortex subventricular zone	Present		3H , 3G
TS21: thalamus mantle layer	Present		3G
TS21: thalamus ventricular layer	Present		3G
TS21: diencephalon	Present	Regionally restricted	3G	Expressed in the eminentia thalami and thalamus.
TS21: hypothalamus	Present	Regionally restricted	3G	Expressed in a part of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		4H
TS23: cerebral cortex ventricular layer	Present		4H
TS23: cortical plate	Weak		4H

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		4K
TS23: cerebral cortex ventricular layer	Present		4K
TS23: cortical plate	Weak		4K

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Scattered	5A	Expressed in a distinctive salt-and-pepper expression pattern in progenitor cells.

Structure	Level	Pattern	Image	Note
TS21: retina	Strong	Regionally restricted	6C	Robust expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	6D	No expression in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present		7C
TS20: hindbrain ventricular layer	Present	Regionally restricted	7C	Expressed in the dorsal hindbrain near the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present		7D
TS20: hindbrain ventricular layer	Present	Regionally restricted	7D	Expressed in the dorsal hindbrain near the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	8C	Expressed in the dorsal progenitor population.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Widespread	8D	Expression became broader, covering most of the dorso-ventral extent, but with weaker expression ventrally.