Gene Expression Data
Assay Details
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Assay
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Reference:
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J:177134
Cossette S, et al., The identification of different endothelial cell populations within the mouse proepicardium. Dev Dyn. 2011 Oct;240(10):2344-53
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5587016
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-CD31 MGI:5451027 may have been used in this assay.
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Results
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Specimen
2A:
embryonic day 9.0
(more  )
Note: 16-18 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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16-18 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: hepatic primordium |
Present |
Regionally restricted |
2A
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Expression is in endothelial cells surrounding the liver bud and in cells spreading out from the bud into the proepicardium.
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| TS14: trunk |
Present |
Regionally restricted |
2A
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Expression is in endothelial cells surrounding the liver bud and in cells spreading out from the bud into the proepicardium.
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| TS14: sinus venosus |
Present |
Regionally restricted |
2A
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Expression is in endothelial cells extending from the sinus venosus into the proepicardium.
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Specimen
2B:
embryonic day 9.5
(more )
Note: 22-24 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22-24 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
2B
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Expression is in endothelial cells.
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| TS15: sinus venosus |
Present |
Regionally restricted |
2B
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Expression is in cells extending from the sinus venosus.
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| TS15: hepatic primordium |
Present |
Regionally restricted |
2B
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Expression is in endothelial cells.
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| TS15: heart |
Present |
Regionally restricted |
2B
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Expression is in endothelial cells in the sinus venosus, absent on the surface of the heart.
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Specimen
2C:
embryonic day 9.75
(more )
Note: 26-28 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Hnf4a (assay MGI:5587029).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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26-28 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Hnf4a (assay MGI:5587029).
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Specimen
3A:
embryonic day 9.0
(more )
Note: 16-18 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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16-18 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: trunk |
Present |
Regionally restricted |
3A
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Expression is endothelial cells in the proepicardium.
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Specimen
3B:
embryonic day 9.5
(more )
Note: 22-24 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22-24 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
3B
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Expression is in endothelial cells.
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Specimen
3C:
embryonic day 9.75
(more )
Note: 26-28 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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26-28 somites. Triple labeled: red - Pecam1; blue - Wt1 (assay MGI:5587024); green - Erg (assay MGI:5587028).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
3C
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Expression is in endothelial cells.
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Specimen
4C:
embryonic day 9.5
(more )
Note: 22-24 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22-24 somites. Triple labeled: red - Pecam1; green - Wt1 (assay MGI:5587024); blue - Prox1 (assay MGI:5587026).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
4C
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Expression is in endothelial cells.
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Specimen
4D:
embryonic day 9.5
(more )
Note: 22-24 somites. Triple labeled: green - Pecam1; blue - Wt1 (assay MGI:5587024); red - Kdr (assay MGI:5587031).
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22-24 somites. Triple labeled: green - Pecam1; blue - Wt1 (assay MGI:5587024); red - Kdr (assay MGI:5587031).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
4D
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Expression is in endothelial cells.
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Specimen
4E:
embryonic day 9.5
(more )
Note: 22-24 somites. Triple labeled: green - Pecam1; blue - Wt1 (assay MGI:5587024); red - Cdh5 (assay MGI:5587032).
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22-24 somites. Triple labeled: green - Pecam1; blue - Wt1 (assay MGI:5587024); red - Cdh5 (assay MGI:5587032).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: proepicardium |
Present |
Regionally restricted |
4E
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Expression is in endothelial cells.
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Specimen
S3B:
embryonic day 14.5
(more )
Note: Double labeled: green - Pecam1; red - Myh11 (assay MGI:5587040).
Specimen
S3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pecam1; red - Myh11 (assay MGI:5587040).
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