Antibody:	anti-tyrosine hydroxylase
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: cervical ganglion	Present		2A	Expressed in the sympathetic neurons in the column-like structure of the sympathetic primordium.

Structure	Level	Pattern	Image	Note
TS20: cervical ganglion	Present		2B	Expressed in the sympathetic neurons in the sympathetic primordium, which was smaller than that of the wild-type.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Strong		3A	Intense expression in the sympathetic neurons.
TS21: common carotid artery	Present	Regionally restricted	3A	Expresse in glomus cells in the peripheral portion of the carotid body rudiment in the carotid artery bifurcation.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Strong		3D	Intense expression in the sympathetic neurons in the superior cervical ganglion, which had an attenuated growth relative to that of the wild-type.
TS21: common carotid artery	Present	Regionally restricted	3D	Expresse in glomus cells occupied a larger portion of the smaller mutant carotid body rudiment in the carotid artery bifurcation.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Strong		4C	Intense expression in the sympathetic neurons in the superior cervical ganglion, which had an attenuated growth relative to that of the wild-type.

Structure	Level	Pattern	Image	Note
TS23: superior cervical ganglion	Present		5B	Expressed in the sympathetic neurons.
TS23: carotid artery	Present	Clusters	5B	Expressed in small clusters of glomus cells of the carotid body rudiment.

Structure	Level	Pattern	Image	Note
TS23: superior cervical ganglion	Present		5D	Expressed in the sympathetic neurons in the superior cervical ganglion, which is hypoplastic compared with that of the wild-type.
TS23: carotid artery	Present	Regionally restricted	5D	Expressed in glomus cells of the carotid body rudiment, which was smaller compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS23: superior cervical ganglion	Present		5E	Expressed in the sympathetic neurons in the superior cervical ganglion, which is hypoplastic compared with that of the wild-type.
TS23: carotid artery	Present	Regionally restricted	5E	Expressed in glomus cells of the carotid body rudiment, which was smaller compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS26: superior cervical ganglion	Present		6B	Expressed in the sympathetic neurons.

Structure	Level	Pattern	Image	Note
TS26: superior cervical ganglion	Present		6E	Expressed in the sympathetic neurons in the superior cervical ganglion, which was markedly reduced in size compared with that of the wild-type.