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Gene Expression Data
Assay Details
Assay
Reference: J:81311 Villegas G, et al., Ontogeny of semaphorins 3A and 3F and their receptors neuropilins 1 and 2 in the kidney. Mech Dev. 2002 Dec;119 Suppl 1:S149-53
Assay type: RNA in situ
MGI Accession ID: MGI:5602970
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Probe: Sema3a cDNA4
Probe preparation: Antisense labelled with digoxigenin RNA
Assay notes: Probes was hydrolyzed to obtain 300-500 bp cRNAs. Fixative was paraformaldehyde and glutaraldehyde. Specimens were processed for beta-galactosidase prior to paraffin embedding.
Results
Specimen 2 E12.5: embryonic day 12.5; Kdrtm1Jrt/Kdr+ (more )
Note: Double labeled: brown/purple - Sema3a; reporter - Kdr (assay MGI:5602974).
Structure Level Pattern Image Note
TS20: metanephros Present Widespread 2_E12.5
Specimen 2 E15: embryonic day 15.0; Kdrtm1Jrt/Kdr+ (more )
Note: Double labeled: brown/purple - Sema3a; reporter - Kdr (assay MGI:5602974).
Structure Level Pattern Image Note
TS23: s-shaped body Present 2_E15 left
TS23: ureteric tip Present 2_E15 left
Specimen 2 E17: embryonic day 17.0; Kdrtm1Jrt/Kdr+ (more )
Note: Double labeled: brown/purple - Sema3a; reporter - Kdr (assay MGI:5602974).
Structure Level Pattern Image Note
TS25: s-shaped body Present 2_E17 left
TS25: ureteric tip Present 2_E17 left
Specimen 2 A: postnatal adult; Kdrtm1Jrt/Kdr+ (more )
Note: Double labeled: brown/purple - Sema3a; reporter - Kdr (assay MGI:5602974).
Structure Level Pattern Image Note
TS28: renal connecting tubule Present Regionally restricted 2_A left Expression is in the luminal side of the collecting and distal tubules.
TS28: renal distal tubule Present Regionally restricted 2_A left Expression is in the luminal side of the collecting and distal tubules.
TS28: glomerular tuft Present Regionally restricted 2_A left Expression is in podocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
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