Probe:	Kgf cdna1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS24: dermis	Strong	Regionally restricted	3a/a*	Expression was strong in the dermis mesenchymal cells.
TS24: epidermis	Absent		3a/a*
TS24: body wall	Strong	Regionally restricted	3a/a*	Expression was in the panniculus carnosus.

Structure	Level	Pattern	Image	Note
TS24: dermis	Strong	Regionally restricted	3b/b*	Expression was strong in the dermis mesenchymal cells.
TS24: anterior abdominal wall muscle	Strong		3b/b*

Structure	Level	Pattern	Image	Note
TS22: dermomyotome	Strong		3c/c*
TS22: trunk mesenchyme	Strong	Regionally restricted	3c/c*	Strong expression was noted in the mesenchyme ventral-lateral to the neural tube.
TS22: dorsal root ganglion	Absent		3c/c*
TS22: spinal cord	Absent		3c/c*

Structure	Level	Pattern	Image	Note
TS24: face mesenchyme	Present	Regionally restricted	3d/d*	Expression was detected in the mesenchymal cells surrounding the vibrissal follicles.

Structure	Level	Pattern	Image	Note
TS22: mammary mesenchyme	Strong		5b/b*	Expression in mammary mesenchyme was in direct continuity with dermal mesenchyme.
TS22: dermis	Strong	Regionally restricted	5b/b*	Expression was in dermal mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: salivary gland mesenchyme	Present		5d/d*

Structure	Level	Pattern	Image	Note
TS24: face mesenchyme	Present	Regionally restricted	6a/a*	Expression was detected in the mesenchyme subadjacent to oral, nasal, and lingual epithelia.
TS24: esophagus mesenchyme	Present		6a/a*
TS24: palate mesenchyme	Present		6a/a*
TS24: pharynx mesenchyme	Present		6a/a*
TS24: perichondrium	Present	Regionally restricted	6a/a*	Expression was detected in the perichondrium surrounding the laryngeal and tracheal cartilages.
TS24: dental papilla	Absent		6a/a*

Structure	Level	Pattern	Image	Note
TS24: esophagus mesenchyme	Strong		6c/c*
TS24: stomach mesenchyme	Strong	Regionally restricted	6c/c*	Forestomach mesenchyme gave a strong signal.
TS24: stomach pyloric region mesenchyme	Weak		6c/c*
TS24: alimentary system smooth muscle	Present	Regionally restricted	6c/c*	Expression was detected in the developing smooth muscle of the stomach and esophagus.

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Strong		6d

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present		6f

Structure	Level	Pattern	Image	Note
TS24: embryo mesenchyme	Present	Regionally restricted		Expression was detected in mesenchymal cells surrounding the thymus and thyroid epithelia.
TS24: aorta	Absent			The vascular smooth muscle of the aorta was devoid of message.
TS24: liver	Trace	Diffuse
TS24: head mesenchyme	Present	Regionally restricted		Expression was detected in the periostium and mesenchymal cells surrounding foci of intra-membranous ossification in the head.

Structure	Level	Pattern	Image	Note
TS24: dermis	Strong		7c
TS24: trunk musculature	Strong	Regionally restricted	7c	Strong signal was seen in the musculature of the body wall.
TS24: alimentary system smooth muscle	Present		7c

Structure	Level	Pattern	Image	Note
TS24: mesenchyme of urogenital sinus	Moderate		8b/b*
TS24: male reproductive system mesenchyme	Present	Regionally restricted	8b/b*	Expression was detected in the mesenchyme surrounding the seminal vesicle.
TS24: urinary system mesenchyme	Weak	Regionally restricted	8b/b*	Expression was detected in the fibroblasts underlying the urothelium.
TS24: detrusor muscle of bladder	Moderate		8b/b*

Structure	Level	Pattern	Image	Note
TS24: urinary system mesenchyme	Present	Regionally restricted	9c/c*	Expression was detected in the mesenchyme surrounding the ureter.
TS24: adrenal gland	Absent		9c/c*
TS24: metanephros	Absent		9c/c*
TS24: trunk mesenchyme	Strong	Regionally restricted	9c/c*	Strong signal was noted in mesenchyme above the adrenal gland and ventral to the vertebrae.

Structure	Level	Pattern	Image	Note
TS24: testis	Absent		10b/b
TS24: male reproductive system	Weak	Regionally restricted	10b/b	Expression was weak in the Wolffian duct. There was no staining in the efferent ducts.

Structure	Level	Pattern	Image	Note
TS24: ovary	Absent

Structure	Level	Pattern	Image	Note
TS22: ovary	Absent

Structure	Level	Pattern	Image	Note
TS24: dermis	Present		10d/d
TS24: limb musculature	Present		10d/d
TS24: limb mesenchyme	Present	Regionally restricted	10d/d	Expression was detected in mesenchyme surrounding cartilage.
TS24: limb cartilage condensation	Absent		10d/d

Structure	Level	Pattern	Image	Note
TS22: dermis	Present
TS22: dermomyotome	Present
TS22: detrusor muscle of bladder	Present
TS22: diaphragm	Present
TS22: esophagus mesenchyme	Present
TS22: eyelid mesenchyme	Present
TS22: genital tubercle mesenchyme	Present
TS22: limb musculature	Present
TS22: lung mesenchyme	Present
TS22: mammary mesenchyme	Present
TS22: mesenchyme of urogenital sinus	Present
TS22: nasal mesenchyme	Present
TS22: primary palate mesenchyme	Present
TS22: salivary gland mesenchyme	Present
TS22: stomach mesenchyme	Present
TS22: tongue muscle	Present
TS22: trachea mesenchyme	Present
TS22: trunk musculature	Present	Regionally restricted		Expression was detected in the body wall muscle (ventral).
TS22: head bone	Present	Regionally restricted		Expression was detected in bone (facial).
TS22: face mesenchyme	Present	Regionally restricted		Expression was detected in eye socket mesenchyme.
TS22: ureter	Present	Regionally restricted		Expression was detected in ureter mesenchyme.
TS22: alimentary system smooth muscle	Present	Regionally restricted		Expression was detected in the esophagus, stomach (foregut and pyloris region) and rectum smooth muscle.
TS22: thyroid gland	Present	Regionally restricted		Expression was detected in the thyroid mesenchyme.
TS22: thymus primordium	Present	Regionally restricted		Expression was detected in the thymus mesenchyme but was absent from the epithelium.
TS22: intestine smooth muscle	Present	Regionally restricted		Expression was detected in duodenum smooth muscle. Small intestine smooth muscle expression was listed as trace.
TS22: hair follicle	Present	Regionally restricted		Expression was detected in dermal papillae and mesenchyme.
TS22: vibrissa follicle	Present	Regionally restricted		Expression was detected in dermal papillae and mesenchyme.
TS22: tooth mesenchyme	Present	Regionally restricted		Expression was detected in tooth surrounding mesenchyme.
TS22: trunk mesenchyme	Present	Regionally restricted		Expression was detected in mesenchyme ventral to vertebrae.
TS22: larynx	Present	Regionally restricted		Expression was detected in larynx mesenchyme.
TS22: perichondrium	Present	Regionally restricted		Expression was detected in the perichondrium associated with limb cartilage, pelvic bone, ribs, vertebrae, and laryngeal cartilage.
TS22: collecting duct	Present	Regionally restricted		Expression was detected in collecting duct mesenchyme.
TS22: axial skeleton thoracic region	Present	Regionally restricted		Expression was detected in rib mesenchyme.
TS22: choroid plexus	Trace
TS22: duodenum mesenchyme	Trace
TS22: inner ear mesenchyme	Trace
TS22: liver	Trace
TS22: pancreas mesenchyme	Trace
TS22: small intestine mesenchyme	Trace
TS22: stomach pyloric region mesenchyme	Trace
TS22: pituitary gland	Trace	Regionally restricted		Expression was trace in pituitary mesenchyme.
TS22: adrenal gland	Absent
TS22: cornea	Absent
TS22: dental papilla	Absent
TS22: dorsal aorta	Absent
TS22: early nephron	Absent
TS22: laryngeal cartilage	Absent
TS22: lens	Absent
TS22: limb cartilage condensation	Absent
TS22: optic nerve	Absent
TS22: rib cartilage condensation	Absent

Structure	Level	Pattern	Image	Note
TS22: male reproductive system mesenchyme	Present	Regionally restricted		Expression was detected in efferent ducts mesenchyme and the Mullerian duct (male) mesenchyme.
TS22: nephric duct	Trace	Regionally restricted		Trace expression was detected in Wolffian duct (male) mesenchyme.
TS22: testis mesenchyme	Trace

Structure	Level	Pattern	Image	Note
TS22: ovary	Absent

Structure	Level	Pattern	Image	Note
TS24: dermis	Present
TS24: detrusor muscle of bladder	Present
TS24: diaphragm	Present
TS24: esophagus mesenchyme	Present
TS24: eyelid mesenchyme	Present
TS24: genital tubercle mesenchyme	Present
TS24: limb musculature	Present
TS24: lung mesenchyme	Present
TS24: mammary mesenchyme	Present
TS24: mesenchyme of urogenital sinus	Present
TS24: nasal mesenchyme	Present
TS24: palate mesenchyme	Present
TS24: salivary gland mesenchyme	Present
TS24: stomach mesenchyme	Present
TS24: tongue muscle	Present
TS24: trachea cartilaginous ring	Present
TS24: trachea mesenchyme	Present
TS24: trunk musculature	Present	Regionally restricted		Expression was detected in body wall muscle (ventral) and fibroblasts.
TS24: head bone	Present	Regionally restricted		Expression was detected in bone (facial).
TS24: alimentary system smooth muscle	Present	Regionally restricted		Expression was detected in the esophagus, stomach (forestomach and pyloris) and rectum smooth muscle.
TS24: face mesenchyme	Present	Regionally restricted		Expression was detected in eye socket mesenchyme.
TS24: ureter	Present	Regionally restricted		Expression was detected in ureter mesenchyme.
TS24: thyroid gland	Present	Regionally restricted		Expression was detected in the thyroid mesenchyme.
TS24: thymus	Present	Regionally restricted		Expression was detected in the thymus mesenchyme but was absent from the epithelium.
TS24: intestine smooth muscle	Present	Regionally restricted		Expression was detected in duodenum smooth muscle. Small intestine smooth muscle expression was listed as trace.
TS24: vibrissa follicle	Present	Regionally restricted		Expression was detected in dermal papillae and mesenchyme.
TS24: tooth mesenchyme	Present	Regionally restricted		Expression was detected in tooth surrounding mesenchyme.
TS24: trunk mesenchyme	Present	Regionally restricted		Expression was detected in mesenchyme ventral to vertebrae.
TS24: larynx	Present	Regionally restricted		Expression was detected in larynx mesenchyme.
TS24: collecting duct	Present	Regionally restricted		Expression was detected in collecting duct mesenchyme.
TS24: perichondrium	Present	Regionally restricted		Expression was detected in the perichondrium associated with limb cartilage, pelvic bone, ribs, vertebrae, and laryngeal cartilage.
TS24: rib	Present	Regionally restricted		Expression was detected in rib mesenchyme but not rib cartilage.
TS24: choroid plexus	Trace
TS24: duodenum mesenchyme	Trace
TS24: inner ear mesenchyme	Trace
TS24: liver	Trace
TS24: small intestine mesenchyme	Trace
TS24: stomach pyloric region mesenchyme	Trace
TS24: pancreas	Trace	Regionally restricted		Expression was trace in pancreas mesenchyme.
TS24: pituitary gland	Trace	Regionally restricted		Expression was trace in pituitary mesenchyme.
TS24: adrenal gland	Absent
TS24: aorta	Absent
TS24: brain	Absent
TS24: cornea	Absent
TS24: dental papilla	Absent
TS24: early nephron	Absent
TS24: laryngeal cartilage	Absent
TS24: lens	Absent
TS24: limb cartilage condensation	Absent
TS24: optic nerve	Absent
TS24: sclera	Absent
TS24: spinal cord	Absent
TS24: hair follicle	Absent			No expression in hair follicles at E16.

Structure	Level	Pattern	Image	Note
TS24: male reproductive system mesenchyme	Present	Regionally restricted		Expression was detected in efferent ducts mesenchyme, seminal vesicle mesenchyme, and Mullerian duct (male) mesenchyme. Trace expression was noted in Wolffian duct mesenchyme.
TS24: seminal vesicle	Present	Regionally restricted		Expression was detected in seminal vesicle mesenchyme.
TS24: testis mesenchyme	Trace

Structure	Level	Pattern	Image	Note
TS24: ovary	Absent