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Gene Expression Data
Assay Details
Assay
Reference: J:98302 Altick AL, et al., EphB receptor tyrosine kinases control morphological development of the ventral midbrain. Mech Dev. 2005 Apr;122(4):501-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5616103
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tuj1 (rabbit polyclonal 802001)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
Results
Specimen 5A: embryonic day 10.5; Ephb3tm1Kln/Ephb3tm1Kln (more )
Note: Double labeled: green - Tubb3; red - Nkx2-2 (assay MGI:5616102).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5A Authors report expression marking location of nIII motor neuron clusters.
Specimen 5B: embryonic day 10.5; Ephb2tm2Paw/Ephb2tm2Paw, Ephb3tm1Kln/Ephb3tm1Kln (more )
Note: Double labeled: green - Tubb3; red - Nkx2-2 (assay MGI:5616102).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5B Expression in nIII motor neuron clusters retains position relative to lateral marker cells.
Specimen 5C: embryonic day 10.5; Ephb3tm1Kln/Ephb3tm1Kln (more )
Note: Double labeled: green - Tubb3; red - Foxa2 (assay MGI:5616106).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5C Authors report expression marking location of nIII motor neuron clusters.
Specimen 5D: embryonic day 10.5; Ephb2tm2Paw/Ephb2tm2Paw, Ephb3tm1Kln/Ephb3tm1Kln (more )
Note: Double labeled: green - Tubb3; red - Foxa2 (assay MGI:5616106).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5D Expression in nIII motor neuron clusters retains position relative to lateral marker cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory