Probe:	betaigH3 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2B	Expressed in mesenchymal decidual cells in the maternal region of future placenta.
TS14: ectoplacental cone	Absent		2B
TS14: labyrinthine zone	Absent		2B

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Present	Regionally restricted	2F	Expressed in the mesenechyme of the cardiac atrioventricular cushions.
TS15: chorionic plate	Present		2F
TS15: trunk mesenchyme	Present	Regionally restricted	2F	Expressed at lower levels in the mesenchyme surrounding the hindgut and cardinal veins.
TS15: yolk sac	Absent		2F

Structure	Level	Pattern	Image	Note
TS16: endocardial cushion	Present	Regionally restricted	3B	Expressed in the endocardial cushion mesenchyme of the atrioventricular canal and in outflow tract cushions cells.
TS16: branchial arch mesenchyme	Present		3B
TS16: outflow tract mesenchyme	Present		3B	Expressed in myocardium.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Regionally restricted	3F	Expressed in the mesenechyme of the cardiac atrioventricular cushions.
TS17: endocardial lining	Absent		3F

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Absent		3J
TS20: pericardium	Present	Regionally restricted	3J	Expressed in the pericardium overlying the ventricle.
TS20: atrioventricular endocardial cushion	Present		3J
TS20: vertebral pre-cartilage condensation	Weak		3J
TS20: endocardial cushion	Strong	Regionally restricted	3J	Expressed in the atrioventricular and in outflow tract cushions. Expression is higher in outflow tract cushions than in the atrioventricular cushions.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Absent		4B
TS22: meninges	Present	Regionally restricted	4B	Expressed in tissues surrounding the spinal cord.
TS22: vertebral cartilage condensation	Present		4B

Structure	Level	Pattern	Image	Note
TS22: endocardial cushion	Strong		4E
TS22: ascending aorta	Present		4E	Proximal aorta.
TS22: heart mesentery	Present	Single cells	4E	Expressed in isolated cardiac fibroblasts around the outflow tract cushions.
TS22: ductus arteriosus	Strong	Regionally restricted	4E	Expressed in the walls of the ductus arteriosus.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Present	Regionally restricted	4H	Expressed in the large septal leaflet mesenchyme, but is largely absent from the lateral leaflet mesenchyme.
TS22: tricuspid valve	Present	Regionally restricted	4H	Expressed in the large septal leaflet mesenchyme, but is largely absent from the lateral leaflet mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: outflow tract	Present		4K' , 4K
TS22: periosteum	Present	Regionally restricted	4K , 4K'	Expressed in the periostium surrounding the rib.
TS22: rib cartilage condensation	Present	Regionally restricted	4K' , 4K	Expressed in the condensed chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Present	Regionally restricted	5B	Expressed in the valve leaflet fibrous mesenchyme. Absent from the papillary muscles attachment site.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	5B inset	Expressed in a restricted pattern.

Structure	Level	Pattern	Image	Note
TS27: heart valve	Present	Regionally restricted		Expressed in the fibrous mesenchyme.