Antibody:	anti-Dab2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present		1A
TS14: rhombomere 6	Present		1A

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Present		1A'
TS14: rhombomere 6	Present		1A'

Structure	Level	Pattern	Image	Note
TS14: rhombomere 5	Strong		1B
TS14: rhombomere 6	Strong		1B
TS14: central nervous system	Present	Regionally restricted	1B	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.
TS14: neural tube	Present	Regionally restricted	1B	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 5	Strong		1C
TS15: rhombomere 6	Strong		1C

Structure	Level	Pattern	Image	Note
TS15: rhombomere 5	Strong		1C'
TS15: rhombomere 6	Strong		1C'

Structure	Level	Pattern	Image	Note
TS15: rhombomere 6	Strong	Uniform	1E

Structure	Level	Pattern	Image	Note
TS15: rhombomere 6	Strong		1F
TS15: rhombomere 5	Weak	Regionally restricted	1F	Expression was restricted to the ventral part.
TS15: central nervous system	Present	Regionally restricted	1F	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.
TS15: neural tube	Present	Regionally restricted	1F	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 6	Strong	Uniform	1G'' , 1G
TS15: rhombomere 5	Weak	Regionally restricted	1G'' , 1G	Expression was restricted to the ventral part.
TS15: central nervous system	Present	Regionally restricted	1G	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.
TS15: neural tube	Present	Regionally restricted	1G	Expression was in a narrow strip of the ventral neural tube extending from the mesencephalic flexure down to the spinal neural tube.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 6	Weak		1H
TS17: rhombomere 5	Absent		1H

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		1I	Expression in the floor plate was initially detected in the rostral part of the spinal cord.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Weak		1I'
TS15: notochord	Weak		1I'

Structure	Level	Pattern	Image	Note
TS16: neural tube floor plate	Present		1J

Structure	Level	Pattern	Image	Note
TS16: neural tube floor plate	Present		1J'

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Weak		1K	Downregulation of the expression in the floorplate occurred in a rostral to caudal direction along the spinal cord.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		1K'	Downregulation of the expression in the floorplate occurred in a rostral to caudal direction along the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Weak		1L

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Absent		1M

Structure	Level	Pattern	Image	Note
TS15: future hindbrain roof plate	Present		2A

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Present		2B' , 2B

Structure	Level	Pattern	Image	Note
TS19: hindbrain roof plate	Present		2C' , 2C

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present	Non-Uniform	2D , 2D'	More intense expression was associated with the apical aspect (luminal surface and associated microvilli) as compared to the basal aspect.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle choroid plexus	Present		2E

Structure	Level	Pattern	Image	Note
TS24: 4th ventricle choroid plexus	Present		2F

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Present	Regionally restricted	2G	The intensity and localization of expression was reversed compared to embryonic choroid plexus. The luminal surface was weaker compared to the basal surface in the adult.

Structure	Level	Pattern	Image	Note
TS19: subcommissural organ primordium	Present		2H , 2H'

Structure	Level	Pattern	Image	Note
TS20: subcommissural organ primordium	Present		2I' , 2I	Expression became progressively more prominent in the subcommissural organ and in the thin processes extending from the subcommissural organ to the posterior commissure. The ventricular aspect showed only weak immunoreactivity.

Structure	Level	Pattern	Image	Note
TS22: subcommissural organ	Present		2J	Expression became progressively more prominent in the subcommissural organ and in the thin processes extending from the subcommissural organ to the posterior commissure. The ventricular aspect showed only weak immunoreactivity.

Structure	Level	Pattern	Image	Note
TS24: subcommissural organ	Present		2K	Expression became progressively more prominent in the subcommissural organ and in the thin processes extending from the subcommissural organ to the posterior commissure. The ventricular aspect showed only weak immunoreactivity.

Structure	Level	Pattern	Image	Note
TS28: subcommissural organ	Absent		2L

Structure	Level	Pattern	Image	Note
TS24: pineal gland	Present		2N

Structure	Level	Pattern	Image	Note
TS28: pineal gland	Absent

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	3D	Immunopositive cells within the neural tube are often closely apposed to blood vessels.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Regionally restricted	3E	Immunopositive cells within the neural tube are often closely apposed to blood vessels.
TS19: blood vessel endothelium	Present		3E	Expression was in the endothelial lining of the brain microvasculature.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Scattered	3F
TS20: blood vessel endothelium	Present		3F	Expression was in the endothelial lining of the brain microvasculature.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	3G	Immunopositive fetal macrophages were closely associated with the roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS24: blood vessel endothelium	Present		3I	Expression was in the endothelial lining of the brain microvasculature.

Structure	Level	Pattern	Image	Note
TS28: blood vessel endothelium	Absent		3J
TS28: brain	Present		3J	Expression was in perivascular cells.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Strong	Scattered	3M