Gene Expression Data
Assay Details
Assay
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Reference:
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J:162095
Shiwaku H, et al., Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J. 2010 Jul 21;29(14):2446-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5638165
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Gene symbol:
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Ufl1
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Gene name:
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UFM1 specific ligase 1
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Antibody:
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C15-01D
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2B upper,middle:
embryonic day 17.0
(more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Specimen
2B upper,middle:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex |
Present |
Regionally restricted |
2B Merge upper
,
2B Maxer middle
,
2B Maxer upper
,
2B Merge middle
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There was expression in Glast-positive radial glia.
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Specimen
2B lower:
embryonic day 17.0
(more )
Note: Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
Specimen
2B lower:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex |
Present |
Regionally restricted |
2B Merge lower
,
2B Maxer lower
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Expression was detected in Sox2-positive neural stem cells.
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Specimen
2C WT:
postnatal week 1
(more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Specimen
2C WT:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
2C WT Maxer
,
2C WT Merge
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Expression was high in Glast-positive Bergmann glia.
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Specimen
2C Atx-KI:
postnatal week 1; Atxn1tm1Hzo/Atxn1 +
(more )
Note: Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
Specimen
2C Atx-KI:
(close )
Genetic Background:
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B6.129S7-Atxn1tm1Hzo
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Mutant Allele(s):
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Atxn1tm1Hzo/Atxn1+
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Age:
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postnatal week 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: green - Ufl1; red - Slc1a3 (assay MGI:5638186).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
2C Atx1-KI Merge
,
2C Atx1-KI Maxer
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Expression was reduced in Bergmann glia in the mutant.
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Specimen
2D WT:
postnatal week 16
(more )
Note: Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
Specimen
2D WT:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
2D WT Maxer
,
2D WT Merge
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Expression was high in Gfap-positive Bergmann glia.
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Specimen
2D Atx1-KI:
postnatal week 16; Atxn1tm1Hzo/Atxn1 +
(more )
Note: Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
Specimen
2D Atx1-KI:
(close )
Genetic Background:
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B6.129S7-Atxn1tm1Hzo
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Mutant Allele(s):
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Atxn1tm1Hzo/Atxn1+
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ufl1; red - Gfap (assay MGI:5638192).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
2D Atx1-KI Maxer
,
2D Atx1-KI Merge
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Expression was reduced in Bergmann glia in the mutant.
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Specimen
2F:
postnatal week 1
(more )
Note: Double labeled: green - Ufl1; red - Pcna (assay MGI:5638205).
Specimen
2F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Pcna (assay MGI:5638205).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
2F Merge
,
2F Maxer
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Expression was detected in PCNA-positive cells.
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Specimen
S1A:
postnatal week 16
(more )
Note: Double labeled: green - Ufl1; red - Calb1 (assay MGI:5638221).
Specimen
S1A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Ufl1; red - Calb1 (assay MGI:5638221).
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Specimen
S1B:
postnatal week 16
(more )
Note: Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
Specimen
S1B:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Ufl1; red - Sox2 (assay MGI:5638190).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
S1B Maxer
,
S1B Merge
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Expression was in Sox2-positive Bergmann glia.
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