Gene Expression Data
Assay Details
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Assay
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Reference:
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J:175242
Togashi H, et al., Nectins establish a checkerboard-like cellular pattern in the auditory epithelium. Science. 2011 Aug 26;333(6046):1144-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5641214
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Gene symbol:
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Nectin1
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Gene name:
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nectin cell adhesion molecule 1
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Results
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Specimen
not shown:
embryonic day 14.0
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
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Expression at junctions between auditory epithelia.
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Specimen
1 Nectin-1:
postnatal day 1
(more )
Note: Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
Specimen
1 Nectin-1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
1_Nectin-1/MyoVIIa
,
1_Nectin-1
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Expression only at boundaries between between hair cells and supporting cells, not at junctions between supporting cells.
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Specimen
3A WT:
postnatal day 1
(more )
Specimen
3A WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B Nectin-1 KO:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Nectin1tm1Ytk/Nectin1tm1Ytk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C Nectin-2 KO:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Nectin2tm1Smu/Nectin2tm1Smu
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
3C Nectin-1
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Authors report expression remained unchanged compared with wild type mice.
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Specimen
3D nectin-3 KO:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Nectin3tm1Ytk/Nectin3tm1Ytk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
3D Nectin-1
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Expression abnormally concentrated at boundaries between hair cells. No expression at junctions between hair cells and supporting cells.
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Specimen
S2A Nectin-1:
embryonic day 16.0
(more )
Note: Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
Specimen
S2A Nectin-1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: organ of Corti |
Present |
Regionally restricted |
S2A Nectin-1/MyoVIIa
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S2A Nectin-1
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Expression at apical surface of auditory epithelium in middle turn of cochlea. Authors report expression at E16 similar to that at P1.
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Specimen
S3A Nectin-1:
embryonic day 16.0
(more )
Note: Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
Specimen
S3A Nectin-1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: organ of Corti |
Present |
Regionally restricted |
S3A Nectin-1/MyoViia
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S3A Nectin-1
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Expression localized on apical side of lateral membranes of hair cells and supporting cells.
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Specimen
S3D Nectin-1:
embryonic day 16.0
(more )
Note: Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
Specimen
S3D Nectin-1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: organ of Corti |
Present |
Regionally restricted |
S3D Nectin-1
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S3D Nectin-1/MyoVIIa
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Expression localized on apical side of lateral membranes of hair cells and supporting cells.
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Specimen
S5A Nectin-1:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Nectin3tm1Ytk/Nectin3tm1Ytk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Nectin1; red - Myo7a (assay MGI:5641213)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
S5A Nectin-1
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S5A Nectin-1/MyoVIIa
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Expression abnormally concentrated at boundaries between hair cells. No expression at junctions between hair cells and supporting cells.
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Specimen
S5B Nectin-1:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Nectin3tm1Ytk/Nectin3tm1Ytk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nectin1; red - F-Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
S5B Nectin-1/F-Actin
,
S5B Nectin-1
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Expression abnormally concentrated at boundaries between hair cells. No expression at junctions between hair cells and supporting cells.
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Analysis Tools