Antibody:	Anti-Emx2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Graded	1a	Expression restricted mainly to telencephallic neuroepithelium. Distribution is graded along anterior-posterior axis, with a caudal-medial maximum and rostral-lateral minimum.
TS20: cerebral cortex marginal layer	Present	Scattered	1a	Intense expression in a few scattered cells in the marginal-most part of the cerebral wall, more abundant at its caudal pole

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Graded	1b	Distribution of expression in telencephallic neuroepithelium is graded along anterior-posterior axis, with a caudal-medial maximum and rostral-lateral minimum.
TS23: cerebral cortex ventricular layer	Present		1b	Expression mainly confined to ventricular zone. Expression in ventricular neuroepithelium.
TS23: cerebral cortex marginal layer	Present	Regionally restricted	1b	Expression in scattered cells in marginal layer. Expression still abundant in cells in marginal-most part of cerebral wall. Faint expression in marginal-most part of the forming cortical plate.
TS23: cortical plate	Absent		1b	Expression absent in transitional field, subplate and cortical plate.
TS23: cortical subplate	Absent		1b	Expression absent in transitional field, subplate and cortical plate.
TS23: hippocampus	Present		1b	Expression in hippocampal anlage.
TS23: telencephalon	Strong	Regionally restricted	1b	Intense expression in numerous cells underneath the pia, in area of secondary proliferative zone for dentate gyrus.

Structure	Level	Pattern	Image	Note
TS26: occipital cortex	Present	Regionally restricted	1c	Expression in parieto-occipital cerebral cortex.
TS26: parietal cortex	Present	Regionally restricted	1c	Expression in parieto-occipital cerebral cortex.
TS26: cerebral cortex ventricular zone	Present	Graded	1c	Expression mainly confined to ventricular zone. Higher expression in single ventricular zones in the caudal-medial than in rostral-lateral telencephalon.
TS26: cerebral cortex marginal zone	Present	Scattered	1c	Expression in scattered cells at outer border of marginal layer.
TS26: neocortex	Present	Regionally restricted	1c	Expression in outer part of neocortical layer I, in an immediately subpial position.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	1d	Expression restricted to scattered cells in outer marginal layer
TS23: cortical plate	Present	Regionally restricted	1d	Expression specifically localized in proximal pieces of apical dendrite of outer cortical plate neurons.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	1d'	Expression restricted to scattered cells in outer marginal layer
TS23: cortical plate	Present	Regionally restricted	1d'	Expression specifically localized in proximal pieces of apical dendrite of outer cortical plate neurons.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Regionally restricted	1e	Expression in presumptive Cajal-Retzius cells.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	1f	Distribution of expression in telecephallic neuroepithelium is graded along medial-lateral axis, with a caudal-medial maximum and rostral-lateral minimum.
TS26: amygdala	Present	Regionally restricted	1f	Expression in medial part of nuclear complex of amygdala.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Present	Regionally restricted	1g	Abundant expression in mitral layer of accessory olfactory bulb. Expression in numerous cells in the subependymal layer; subependymal domain stops at boundary between bulbar and retrobulbar zones. Low level of expression in proliferative layer.

Structure	Level	Pattern	Image	Note
TS26: hippocampus region	Present	Regionally restricted	1h	Expression in primary neuroepithelium as well as in dentate gyrus anlage.
TS26: hippocampus ventricular layer	Present		1h
TS26: dentate gyrus	Present		1h	Abundant expression in coalescing dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		1i	Persistent expression in dentate gyrus.
TS28: dentate gyrus molecular layer	Strong	Scattered	1i	Intense expression in single cells in the marginal-most part of both area dentata outer molecular layer.
TS28: hippocampus region	Present	Regionally restricted	1i	Expression in the hilar region between dentate gyrus and hippocampus proper.
TS28: hippocampus	Present	Scattered	1i	Expression in scattered cells in the region of the hippocampus. Authors report that level of expression in ventricular neuroepithelium decayed quickly after birth
TS28: hippocampus molecular layer	Strong	Single cells	1i	Intense expression in single cells in hippocampal stratum lacunosum-moleculare.
TS28: hippocampus stratum lacunosum	Strong	Single cells	1i	Intense expression in single cells in hippocampal stratum lacunosum-moleculare.
TS28: telencephalon	Present	Regionally restricted	1i

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2a	Expression in a few marginally located cells in the ventral mesencephalon.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	2b	Expression in ventral midbrain in a group of cells in medial part of developing tegmentum.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	2c	Intense expression in the mammillary area.
TS20: midbrain	Present	Regionally restricted	2c	Enlarged expression domain, extended rostro-caudally along almost all of midbrain.
TS20: midbrain roof plate	Present	Regionally restricted	2c	Expression in a cohort of cells located in an intermediate position between ventricular and marginal edges of dorsal mesencephalic wall. Expression in a row of cells throughout the entire developing tectum.

Structure	Level	Pattern	Image	Note
TS26: tectum	Present	Scattered	2d	Expression detected in three deeper longitudinal stripes. Expression in scattered cells located in all 3 presumptive strata grisea of the tectum.

Structure	Level	Pattern	Image	Note
TS14: olfactory placode	Present		3a
TS14: head mesenchyme	Present	Regionally restricted	3a	Expression detected in scattered cells located in mesenchyme between olfactory pit and telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present		3b
TS17: olfactory epithelium	Present		3b	Expression in olfactory epithelium.
TS17: head mesenchyme	Present	Regionally restricted	3b
TS17: telencephalon	Present	Regionally restricted	3b

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	3c	Expression detected in scattered cells located in mesenchyme between olfactory pit and telencephalic vesicle.
TS20: telencephalon	Present	Regionally restricted	3c	Expression in two rows of cells on both sides of boundary between mesenchyme and telencephalic walls, along both alar and basal surfaces of telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		3d	Expression in precursors of both main olfactory epithelium and vomeronasal organ, just budding off enlarging nasal cavity.
TS20: vomeronasal organ epithelium	Present		3d	Expression in precursors of both main olfactory epithelium and vomeronasal organ, just budding off enlarging nasal cavity.
TS20: head mesenchyme	Present	Regionally restricted	3d	Expression in scattered cells in mesenchyme between main olfactory epithelium and vomeronasal organ.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	3e	Expression in main olfactory epithelium restricted to nuclei located in the outer half of epithelium, possibly basal cells (olfactory epithelium stem cells) and young sensory neurons. No expression in mature neurons located in luminal half of epithelium.

Structure	Level	Pattern	Image	Note
TS13: head	Present	Regionally restricted		Expression in anterior headfold, more intense laterally than medially.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted		Expression in the mammillary area.

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted		Expression in presumptive cerebral cortex, throughout entire width of telencephalic wall, more abundant dorso-caudally than ventro-rostrally. Almost undetectable in developing ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted		Expression only a few scattered cells in the first rhombomere.

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Regionally restricted		Expression in numerous cells in the area of basal telencephalon.

Structure	Level	Pattern	Image	Note
TS24: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted		Expression in presumptive interneurons and glial cells of the internal granular layer.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Present
TS28: mammillary body	Present			Expression in the mammillary area and tegmentum persistent after birth.
TS28: midbrain tegmentum	Present			Expression in the mammillary area and tegmentum persistent after birth.