Probe:	Irx4 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present			Expression is present in the cardiogenic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2E	Expression is present in the middle portion of the heart, in what is referred to as the ventricular segment. This region gives rise to the lower part of the atrial chamber that is derived from the atrioventricular canal, as well the ventricular chamber.

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present		2G
TS15: outflow tract	Present	Regionally restricted	2G	Expression is present in the proximal tract.
TS15: primitive ventricle cardiac muscle	Present		2G	Expression is present in the inner curvature and ventricular myocardium.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present			Expression is present in the myocardium.
TS17: endocardial cushion	Absent			No expression in the antrioventricular cushions.

Structure	Level	Pattern	Image	Note
TS19: atrioventricular canal	Present		5E , 5B	Expression is present in the myocardium.
TS19: endocardial cushion	Absent		5B , 5E	No expression in the antrioventricular cushions.
TS19: ventricle cardiac muscle	Present	Non-Uniform	5B , 5E	Expression is lower in the trabecules than in the compact myocardium.

Structure	Level	Pattern	Image	Note
TS19: outflow tract	Absent		5G	No expression in the future aorta or pulmonary trunc.
TS19: ventricle cardiac muscle	Present	Non-Uniform	5G	Expression is lower in the trabecules than in the compact myocardium.