Probe:	Irx6 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: endocardial lining	Weak		1B Irx6
TS20: skin	Present		1B Irx6
TS20: hindbrain	Present	Regionally restricted	1B Irx6
TS20: spinal cord	Present	Regionally restricted	1B Irx6

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	2F	Expression is present in spots where cranial nerves III and IV will develop.
TS19: midbrain floor plate	Present	Regionally restricted	2F	Expression is present in spots where cranial nerves III and IV will develop.
TS19: metencephalon basal plate	Present	Regionally restricted	2F	Expression is present in the motor part of the pons, where the motor nuclei of the 7th cranial nerve will develop.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	2L	Expression is present in motor neurons.

Structure	Level	Pattern	Image	Note
TS19: inner ear	Absent		2R	No expression is seen in the otic vesicle.
TS19: acoustic ganglion	Present		2R	Expression is present in the complex.
TS19: facial ganglion	Present		2R	Expression is present in the complex.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar	Absent		3M
TS21: upper jaw molar	Absent		3M

Structure	Level	Pattern	Image	Note
TS15: limb	Weak

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted		Expression is present in the proximal limb.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present
TS21: hindlimb interdigital region mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region mesenchyme	Present
TS22: hindlimb interdigital region mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present
TS17: lung	Absent

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Absent
TS19: gonad	Absent
TS19: trachea	Absent			There is no expression in the larygo-tracheal groove.
TS19: metencephalon basal plate	Present	Regionally restricted		Expression is present in the pons.
TS19: acoustic ganglion	Present			Expression is present in the complex.
TS19: facial ganglion	Present			Expression is present in the complex.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted		Expression is present in motor neurons.
TS21: spinal cord ventricular layer	Weak
TS21: neural retinal epithelium	Present	Regionally restricted		Expression is present in the marginal zone of the neural retina.
TS21: mammary gland primordium	Present
TS21: vibrissa follicle	Absent
TS21: epidermis	Weak	Regionally restricted		Expression is found in the limb and thorax.
TS21: dermis	Absent
TS21: gonad	Absent
TS21: alimentary system epithelium	Absent			There is no expression in the endoderm of the gastro-intestinal tract.
TS21: endocardial tissue	Present	Regionally restricted		There is expression in the atrial and ventricular endodcardium, but not in the endocardium of the cushions.
TS21: atrium endocardial lining	Present
TS21: ventricle endocardial lining	Present