Probe:	Irx4 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: extraembryonic component cavity	Present	Regionally restricted		Expression is present in the chorion.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present			Expression is present in the cardiac crescent.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present

Structure	Level	Pattern	Image	Note
TS20: heart	Present		1B Irx4
TS20: skin	Present		1B Irx4
TS20: hindbrain	Present		1B Irx4

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate	Present	Regionally restricted	2C	Expression is seen at the level of the sulcus limitans and in two spots in the motor part of the pons.
TS19: trigeminal ganglion	Absent		2C	No expression is seen in the 5th cranial nerve.
TS19: midbrain	Absent		2C

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Absent		2I
TS19: surface ectoderm	Present		2I	Expression is present in the epidermis.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Absent		2O
TS19: facial ganglion	Absent		2O
TS19: inner ear	Absent		2O	No expression was seen in the otic vesicle.

Structure	Level	Pattern	Image	Note
TS21: epidermis	Strong	Widespread	3E
TS21: dermis	Absent		3E
TS21: mammary gland primordium	Absent		3E	There is virtually no expression in the mammary gland.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar	Absent		3J
TS21: upper jaw molar	Absent		3J

Structure	Level	Pattern	Image	Note
TS15: limb	Absent

Structure	Level	Pattern	Image	Note
TS17: limb	Absent

Structure	Level	Pattern	Image	Note
TS19: limb	Absent

Structure	Level	Pattern	Image	Note
TS20: limb	Absent

Structure	Level	Pattern	Image	Note
TS21: limb	Absent

Structure	Level	Pattern	Image	Note
TS22: limb	Absent

Structure	Level	Pattern	Image	Note
TS19: trachea	Absent		5C	There is no expression in the laryncho-tracheal groove.

Structure	Level	Pattern	Image	Note
TS21: left ventricle cardiac muscle	Present		5K	Expression is present in the compact myocardium.
TS21: right ventricle cardiac muscle	Present		5K	Expression is present in the compact myocardium.
TS21: atrioventricular canal	Present	Regionally restricted	5K	Expression is present in the myocardium.
TS21: lung epithelium	Absent		5K
TS21: lung mesenchyme	Absent		5K

Structure	Level	Pattern	Image	Note
TS17: lung	Absent

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present
TS19: gonad	Absent
TS19: pancreas primordium	Absent

Structure	Level	Pattern	Image	Note
TS21: vibrissa follicle	Present			Expression is present in the germinal matrix.
TS21: neural retinal epithelium	Present	Regionally restricted		Expression is present in the marginal zone.
TS21: alimentary system epithelium	Absent			There is no expression in the endoderm of the gastro-intestinal tract.
TS21: spinal cord ventricular layer	Weak
TS21: spinal cord lateral wall	Present	Regionally restricted		Expression is present in motor neurons.
TS21: cap mesenchyme	Weak			There is weak expression in the blastema.
TS21: gonad	Absent