Gene Expression Data
Assay Details
Assay
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Reference:
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J:50943
Cho EA, et al., TCF-4 binds beta-catenin and is expressed in distinct regions of the embryonic brain and limbs. Mech Dev. 1998 Sep;77(1):9-18
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5689859
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Gene symbol:
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Tcf7l2
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Gene name:
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transcription factor 7 like 2, T cell specific, HMG box
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Probe:
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mTcf-4 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Embryos were post-fixed in 0.2% glutaraldehyde/4% paraformaldehyde.
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Results
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Specimen
4A:
embryonic day 7.5
(more )
Specimen
4A:
(close )
Genetic Background:
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either: CD-1 or FVB
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Age:
|
embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo endoderm |
Present |
Regionally restricted |
4A
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Expression is at the anterior endoderm.
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Specimen
4B:
embryonic day 7.0
(more )
Specimen
4B:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 7.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo endoderm |
Weak |
Regionally restricted |
4B
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Expression is at the most anterior endoderm.
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Specimen
4C:
embryonic day 10.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
4E:
embryonic day 8.5
(more )
Note: Bisected embryo
Specimen
4E:
(close )
Genetic Background:
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either: CD-1 or FVB
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Age:
|
embryonic day 8.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Bisected embryo
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Specimen
4F:
embryonic day 13.5
(more )
Specimen
4F:
(close )
Genetic Background:
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either: CD-1 or FVB
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
4G:
embryonic day 11.5
(more )
Specimen
4G:
(close )
Genetic Background:
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either: CD-1 or FVB
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
4H:
(close )
Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B:
embryonic day 11.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
5B
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Expression is at the distal end and the proximal region.
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TS19: hindlimb bud |
Present |
Regionally restricted |
5B
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Expression is at the proximal region.
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: digit mesenchyme |
Present |
Regionally restricted |
5C
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Expression is in the area of tarsal/carpal condensations.
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Specimen
5D:
embryonic day 13.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5E:
embryonic day 13.5
(more )
Specimen
5E:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: limb mesenchyme |
Present |
Regionally restricted |
5E
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Expression is in mesenchymal cells surrounding the differentiating cartilage.
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TS21: limb digit |
Present |
Regionally restricted |
5E
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Expression is in bones at the distal end of the digits.
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Specimen
not shown E9.5:
embryonic day 9.5
(more )
Specimen
not shown E9.5:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
Genetic Background:
|
either: CD-1 or FVB
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
|
Expression is at the tip of the distal region.
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Specimen
not shown Sey-/+:
embryonic day 10.5; Pax6Sey/Pax6 +
(more )
Specimen
not shown Sey-/+:
(close )
Genetic Background:
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involves: CD-1
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
not shown Sey-/-:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Specimen
not shown Sey-/-:
(close )
Genetic Background:
|
involves: CD-1
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
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