Antibody:	anti-Prox1 (#11-002)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Widespread	1A'	Expression in the subventricular and mantle zones.
TS22: thalamus mantle layer	Present		1A'	Expression in the dorsal thalamus.
TS22: amygdala	Present	Regionally restricted	1A'	Expression in the mantle zone.
TS22: hippocampus	Present	Regionally restricted	1A'	Expression in dentate gyrus neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Widespread	1B'	Expression in the subventricular and mantle zones.
TS22: thalamus mantle layer	Present	Regionally restricted	1B'	Expression in the dorsal thalamus.
TS22: prethalamus	Absent		1B'	Other experiments showed the mRNA is present at this time.
TS22: hypothalamus subventricular zone	Present	Regionally restricted	1B'	Expression in the subventricular zone of the preoptic area.
TS22: amygdala	Present	Regionally restricted	1B'	Expression in the mantle zone.
TS22: hippocampus	Present	Regionally restricted	1B'	Expression in dentate gyrus neuroepithelium.
TS22: thalamus subventricular zone	Present	Regionally restricted	1B'	Expression in the paraventricular nucleus.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Widespread	1C'	Expression in the subventricular and mantle zones.
TS22: thalamus mantle layer	Present		1C'	Expression in the dorsal thalamus.
TS22: epithalamus mantle layer	Present		1C'
TS22: prethalamus	Absent		1C'	Other experiments showed the mRNA is present at this time.
TS22: hippocampus	Present	Regionally restricted	1C'	Expression in dentate gyrus neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: epithalamus mantle layer	Present		1D'
TS22: prethalamus	Absent		1D'	Other experiments showed the mRNA is present at this time.
TS22: hypothalamus subventricular zone	Present	Regionally restricted	1D'	Expression in the subventricular zone of the arcuate nucleus.
TS22: hippocampus	Present	Regionally restricted	1D'	Expression in dentate gyrus neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: tectum	Present	Regionally restricted	1E'	Expression in the subventricular zone of the colliculus.
TS22: pretectum	Present	Widespread	1E'	Expression in the mantle zone (and subventricular zone).
TS22: midbrain tegmentum	Present	Regionally restricted	1E'	Expression in the subventricular zone.
TS22: pons	Present	Regionally restricted	1E'	Expression in the subventricular zone of the pontine neuroepithelium.
TS22: prethalamus	Absent		1E'	Other experiments showed the mRNA is present at this time.
TS22: hypothalamus subventricular zone	Present	Regionally restricted	1E'	Expression in the subventricular zone of the preoptic area and the arcuate nucleus
TS22: thalamus mantle layer	Present	Regionally restricted	1E'	Expression in the dorsal thalamus.
TS22: hippocampus	Present	Regionally restricted	1E'	Expression in dentate gyrus neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted		Expression is present in the subventricular zone.
TS22: pons	Present	Regionally restricted		Expression is present in the subventricular zone.