Gene Expression Data
Assay Details
Assay
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Reference:
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J:117269
Lavado A, et al., Prox1 expression patterns in the developing and adult murine brain. Dev Dyn. 2007 Feb;236(2):518-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5693875
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Antibody:
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anti-NH-Prox1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3B:
embryonic day 16.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3D:
embryonic day 16.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: prethalamus |
Absent |
|
3D
|
There is no protein expression of in the area of the prethalamus (but there is mRNA present).
|
Specimen
3D inset:
embryonic day 16.5
(more )
Specimen
3D inset:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hippocampus |
Present |
Regionally restricted |
3D inset
|
Expression is present in the dentate migratory stream.
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Specimen
3E:
embryonic day 16.5
(more )
Note: Double labeled: brown - Prox1 protein; blue - Prox1 RNA (assay MGI:5693787).
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Prox1 protein; blue - Prox1 RNA (assay MGI:5693787).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: thalamus |
Present |
Regionally restricted |
3E
|
Areas of expression include the paraventricular nucleus.
|
TS24: prethalamus |
Absent |
|
3E
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There is no protein expression of in the area of the prethalamus (but there is mRNA present).
|
TS24: amygdala |
Present |
Regionally restricted |
3E
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Expression is present in the subventricular zone.
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Specimen
3G:
embryonic day 16.5
(more )
Specimen
3G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3G inset:
embryonic day 16.5
(more )
Specimen
3G inset:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3H:
embryonic day 16.5
(more )
Note: Double labeled: brown - Prox1 protein; blue - Prox1 RNA (assay MGI:5693787).
Specimen
3H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Prox1 protein; blue - Prox1 RNA (assay MGI:5693787).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: amygdala |
Present |
Regionally restricted |
3H
|
Expression is present in the subventricular zone.
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TS24: hypothalamus |
Present |
Regionally restricted |
3H
|
Expression is present in the ventromedial hypothalmus.
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TS24: thalamus |
Present |
Regionally restricted |
3H
|
Although there is protein expression elsewhere in the thalamus, there is no expression in the subgeniculate nucleus.
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TS24: zona incerta |
Absent |
|
3H
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There is no expression in the ventral portion of the zona incerta. This area does have mRNA expression.
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Specimen
3K:
embryonic day 16.5
(more )
Specimen
3K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4B:
embryonic day 18.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: thalamus |
Strong |
Regionally restricted |
4B
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Areas of expression include the laterodorsal and ventrolateral thalamus.
|
TS26: prethalamus |
Absent |
|
4B
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There is no expression present in the area surrounding the prethalamus.
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TS26: dentate gyrus |
Present |
|
4B
|
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Specimen
4D:
embryonic day 18.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: thalamus |
Strong |
Regionally restricted |
4D
|
Although protein is present elsewhere in the thalamus, it is not found in the subgeniculate nucleus.
|
TS26: zona incerta |
Absent |
|
4D
|
No expression in the ventral zona incerta.
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TS26: dentate gyrus |
Present |
|
4D
|
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Specimen
4F:
embryonic day 18.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4G:
embryonic day 18.5
(more )
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4H:
embryonic day 18.5
(more )
Specimen
4H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: tectum |
Present |
Regionally restricted |
4H
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Expression is present along the mantle zone of the colliculus, as well as the subventricular zone.
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