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Gene Expression Data
Assay Details
Assay
Reference: J:211182 Deng M, et al., Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014 May;15(1):31-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697725
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2B/C: embryonic day 9.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Strong Widespread 2B , 2C Expressed in all migrating and delaminated cochleovestibular ganglion cells as well as the facial ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
TS15: otic pit epithelium Present Regionally restricted 2B , 2C Expressed in the epithelial cochleovestibular ganglion precursors.

Specimen 2E/F: embryonic day 10.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS17: acoustic ganglion Strong Widespread 2F , 2E Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
TS17: facial ganglion Strong Widespread 2E , 2F Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
TS17: otocyst epithelium Present Regionally restricted 2E , 2F Expressed in the epithelial cochleovestibular ganglion precursors.

Specimen 2H/I: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS19: acoustic ganglion Strong Widespread 2H , 2I Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
TS19: facial ganglion Strong Widespread 2I , 2H Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.

Specimen 2K/L: embryonic day 12.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS20: vestibulo-cochlear ganglion Strong Widespread 2L , 2K Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
TS20: facial ganglion Strong Widespread 2L , 2K Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.

Specimen 2N/O: embryonic day 13.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS21: cochlear ganglion Present Widespread 2N , 2O Coexpressed with Pou4f1.
TS21: vestibular ganglion Present Widespread 2N , 2O Coexpressed with Pou4f1.
TS21: vestibulo-cochlear ganglion Present Widespread 2O , 2N Expression level was lower in the spiral ganglion than in the vestibular ganglion.

Specimen 2Q/R: embryonic day 15.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS23: cochlear ganglion Strong 2Q , 2R
TS23: cochlea Present Regionally restricted 2Q , 2R Expressed in the cochlear sensory region.

Specimen 2T/U: embryonic day 17.5 (more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Structure Level Pattern Image Note
TS25: cochlear ganglion Strong Widespread 2T , 2U Expressed in almost all cells.
TS25: cochlea Present Regionally restricted 2U , 2T Expressed in the cochlear sensory region.

Specimen 3J: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 3J Expressed in the cochleovestibular ganglion cells.
TS17: facial ganglion Present 3J

Specimen 3K: embryonic day 10.5; Neurod1tm1Mjts/Neurod1tm1Mjts (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 3K A severe loss of the cochleovestibular ganglion cells was observed. Expressed in the remaining cochleovestibular ganglion cells.
TS17: facial ganglion Present 3K

Specimen 3L: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 3L Expressed in the cochleovestibular ganglion cells.
TS17: facial ganglion Present 3L

Specimen 3M: embryonic day 10.5; Pou4f1tm1Nat/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 3M Expressed in the cochleovestibular ganglion cells, same as the control.
TS17: facial ganglion Present 3M


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory