Gene Expression Data
Assay Details
Assay
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Reference:
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J:211182
Deng M, et al., Comparative expression analysis of POU4F1, POU4F2 and ISL1 in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2014 May;15(1):31-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5697725
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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clone 39.4D5
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
2B/C:
embryonic day 9.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2B/C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: facio-acoustic preganglion complex |
Strong |
Widespread |
2B
,
2C
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Expressed in all migrating and delaminated cochleovestibular ganglion cells as well as the facial ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
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TS15: otic pit epithelium |
Present |
Regionally restricted |
2B
,
2C
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Expressed in the epithelial cochleovestibular ganglion precursors.
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Specimen
2E/F:
embryonic day 10.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2E/F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: acoustic ganglion |
Strong |
Widespread |
2F
,
2E
|
Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
|
TS17: facial ganglion |
Strong |
Widespread |
2E
,
2F
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Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
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TS17: otocyst epithelium |
Present |
Regionally restricted |
2E
,
2F
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Expressed in the epithelial cochleovestibular ganglion precursors.
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Specimen
2H/I:
embryonic day 11.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2H/I:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: acoustic ganglion |
Strong |
Widespread |
2H
,
2I
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Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
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TS19: facial ganglion |
Strong |
Widespread |
2I
,
2H
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Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
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Specimen
2K/L:
embryonic day 12.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2K/L:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: vestibulo-cochlear ganglion |
Strong |
Widespread |
2L
,
2K
|
Expressed in all migrating and delaminated cochleovestibular ganglion cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
|
TS20: facial ganglion |
Strong |
Widespread |
2L
,
2K
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Expressed in all cells. Broader expression domain than that of Pou4f1, and encompassed all Pou4f1-positive cells.
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Specimen
2N/O:
embryonic day 13.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2N/O:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
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Specimen
2Q/R:
embryonic day 15.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2Q/R:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
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Specimen
2T/U:
embryonic day 17.5
(more )
Note: Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
Specimen
2T/U:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Double labeled: green - Isl1; red - Pou4f1 (assay MGI:5697719).
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Specimen
3J:
embryonic day 10.5
(more )
Specimen
3J:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3K:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
3L:
embryonic day 10.5
(more )
Specimen
3L:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3M:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Pou4f1tm1Nat/Pou4f1tm1Nat
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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