Antibody:	anti-Lhx1 (C-20; sc-19341)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Present	Widespread	1A	Expression is throughout the visceral endoderm overlying the epiblast.
TS9: primitive endoderm	Present	Widespread	1A	Expression is throughout the visceral endoderm overlying the epiblast.
TS9: embryo mesoderm	Present	Single cells	1A	Expression is seen in a small number of cells at the proximal rim of the posterior epiblast (emerging from the primitive streak).

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Widespread	1B	Expression is present in mesoderm ingressing along the length of the primitive streak, as well as in distal tip cells.
TS11: mesendoderm	Present		1B	Expression is present in anterior midline mesendoderm.

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Strong	Regionally restricted	1C lower , 1C upper	There is strong expression in the ciliated ventral cells.

Structure	Level	Pattern	Image	Note
TS11: node	Strong		1D upper
TS11: embryo mesoderm	Strong	Regionally restricted	1D upper	Robust expression is seen at the midline.
TS11: head mesenchyme	Present		1D upper
TS11: heart	Present	Regionally restricted	1D upper	Expression is detectable in the cardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS11: node	Strong		1D lower
TS11: embryo mesoderm	Strong	Regionally restricted	1D lower	Robust expression is seen at the midline.
TS11: head mesenchyme	Present		1D lower
TS11: heart	Present	Regionally restricted	1D lower	Expression is detectable in the cardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present		1E Control 3D	Expression is seen in the visceral endoderm.
TS11: embryo	Present		1E Control 3D	Expression is seen in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present		1E Control 2D	Expression is seen in the visceral endoderm.
TS11: embryo	Present		1E Control 2D	Expression is seen in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1E Eomes delta Epi 3D	Expression is eliminated throughout the epiblast in the mutant.
TS11: primitive endoderm	Present		1E Eomes delta Epi 3D	The viseral endoderm is genetically wild type in these animals; it retains expression.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1E Eomes delta Epi 2D	Expression is eliminated throughout the epiblast in the mutant.
TS11: primitive endoderm	Present		1E Eomes delta Epi 2D	The viseral endoderm is genetically wild type in these animals; it retains expression.

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present		S2F Control 2D
TS11: embryo mesoderm	Present		S2F Control 2D

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Present		S2F Control 3D
TS11: embryo mesoderm	Present		S2F Control 3D

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Absent		S2F Lhx1 delta Epi 2D
TS11: embryo mesoderm	Absent		S2F Lhx1 delta Epi 2D

Structure	Level	Pattern	Image	Note
TS11: definitive endoderm	Absent		S2F Lhx1 delta Epi 3D
TS11: embryo mesoderm	Absent		S2F Lhx1 delta Epi 3D