Antibody:	C124
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were described C124 MGI:5703952 and C199 MGI:5523007. Either could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS22: liver	Absent		1_right panel
TS22: spinal cord	Absent		1_right panel
TS22: lateral ganglionic eminence	Strong		1_right panel
TS22: medial ganglionic eminence	Strong		1_right panel
TS22: nose	Strong	Regionally restricted	1_right panel	Strong expression was detected in the ecodermal cells of the frontonasal prominence.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2A , 2B	Expression was detected in the lateral ganglionic eminence.
TS17: optic stalk	Present		2A , 2C
TS17: diencephalon	Present	Regionally restricted	2A , 2D	Expression was detected in ventral thalamus but terminates abruptly at the zona limitans.
TS17: forebrain	Present	Regionally restricted	2A	Expression was detected in 2 domains. The rostral domain (I) and caudal domain (II) separated by a strip of negative tissue called the optoeminential zone.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	3_E12.5 , 3_E12.5 high mag	Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
TS20: prethalamus	Present	Regionally restricted	3_E12.5	Expression was detected in the ventral thalamus and terminated abruptly at the zona limitans.
TS20: lateral ganglionic eminence	Present		3_E12.5
TS20: cerebral cortex	Absent		3_E12.5

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	3_E13.5	Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
TS21: prethalamus	Present	Regionally restricted	3_E13.5	Expression was detected in the ventral thalamus and terminated abruptly at the zona limitans.
TS21: lateral ganglionic eminence	Present		3_E13.5
TS21: cerebral cortex	Absent		3_E13.5
TS21: zona limitans intrathalamica	Absent		3_E13.5

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	3_E14.5	Expression was in 2 regions. Caudal- extends from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus. Rostral- from the lateral ganglionic eminence to the optic stalk.
TS22: prethalamus	Present	Regionally restricted	3_E14.5	Expression was detected in the ventral thalamus and terminated abruptly at the zona limitans.
TS22: lateral ganglionic eminence	Present		3_E14.5
TS22: cerebral cortex	Absent		3_E14.5
TS22: dorsal thalamus	Absent		3_E14.5
TS22: zona limitans intrathalamica	Absent		3_E14.5

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	4_E16.5 Forebrain	Expression remains strong in the rostral expression domains, but begins to weaken in the more caudal domains.
TS24: prethalamus	Weak		4_E16.5 Forebrain
TS24: dorsal thalamus	Present		4_E16.5 Forebrain
TS24: anterior entopeduncular area	Present		4B , 4_E16.5 Forebrain
TS24: main olfactory bulb	Present		4_E16.5 Forebrain , 4C
TS24: cerebral cortex marginal layer	Present	Regionally restricted	4_E16.5 Forebrain , 4C , 4D	Expression was in dispersed cells, continuous with the positive staining in the lateral ganglionic eminence and olfactory bulb. Staining in the cerebral cortex was only a fraction of the staining in the subventricular zone of the ganglionic eminence.
TS24: cerebral cortex subventricular zone	Present	Regionally restricted	4_E16.5 Forebrain , 4C , 4D	Expression was in dispersed cells, continuous with the positive staining in the lateral ganglionic eminence and olfactory bulb. Staining in the cerebral cortex was only a fraction of the staining in the subventricular zone of the ganglionic eminence.
TS24: cerebral cortex intermediate zone	Present	Scattered	4D , 4_E16.5 Forebrain
TS24: cortical plate	Present	Scattered	4D , 4_E16.5 Forebrain
TS24: cortical subplate	Present	Scattered	4D , 4_E16.5 Forebrain
TS24: ganglionic eminence	Present	Regionally restricted	4C , 4_E16.5 Forebrain	Expression was detected in all cells of the subventricular zone of the ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present	Regionally restricted	5B , 5_P17	Expression was detected in the periventricular region of the striatum.
TS28: telencephalon	Present	Regionally restricted	5_P17 , 5C	Expression was detected in cells that lie between the cerebral cortex and the striatum.
TS28: main olfactory bulb	Present		5_P17 , 5D
TS28: caudate-putamen	Present	Regionally restricted	5_P17	Expression was detected in the subventricular zone of the caudoputamen complex.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present	Regionally restricted	5E , 5_Adult	Expression was detected in the periventricular region of the striatum.
TS28: telencephalon	Present	Regionally restricted	5F , 5_Adult	Expression was detected in cells that lie between the cerebral cortex and the striatum.
TS28: main olfactory bulb	Present		5G , 5_Adult
TS28: caudate-putamen	Present	Regionally restricted	5_Adult	Expression was detected in the subventricular zone of the caudoputamen complex.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch ectoderm	Present	Regionally restricted	6_E10.5 1st branchial arch	Expression in the ectoderm outlines the bud with maximal expression in the most distal ectoderm.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud ectoderm	Present	Regionally restricted	6_E10.5 hindlimb bud	Expression in the ectoderm outlines the bud with maximal expression in the most distal ectoderm.

Structure	Level	Pattern	Image	Note
TS20: surface ectoderm of genital tubercle	Present	Regionally restricted	6_E12.5 GT	Expression was detected in the distal ectoderm of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS20: tail surface ectoderm	Present		6_E12.5 tail

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Strong	Regionally restricted	7_Dlx2 bottom , 7_Dlx2 top , 7_Dlx2 middle	Strong expression was detected in the subventricular zone of the medial ganglionic eminence. Moderate expression was detected in the submantle. No expression was in the ganglionic mantle.
TS20: prethalamus	Moderate		7_Dlx2 top
TS20: hypothalamus	Moderate	Regionally restricted	7_Dlx2 top	Expression was detected in the preoptic area and the suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS26: medial ganglionic eminence	Present	Regionally restricted		All of the cells in the medial ganglionic eminence subventricular layer are positive.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present	Regionally restricted	9D	Comparing the immunopositive cells with BrdU staining, there is overlap of immunopositive cells with cells that are undergoing S-phase of the cell cycle in the medial ganglionic eminence.