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Gene Expression Data
Assay Details
Assay
Reference: J:203063 Vestin A, et al., The tumor suppressor Chd5 is induced during neuronal differentiation in the developing mouse brain. Gene Expr Patterns. 2013 Dec;13(8):482-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5705558
Gene symbol: Chd5
Gene name: chromodomain helicase DNA binding protein 5
Antibody: Chd5 antibody (M-182)
Detection system: Alexa Fluor 594
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 1A merge , 1A Chd5 Expression in ventral rhombencephalon and posterior mesencephalon.
TS17: midbrain Present Regionally restricted 1A merge , 1A Chd5 Expression in ventral rhombencephalon and posterior mesencephalon.
Specimen S2: embryonic day 10.5 (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted S2_merge , S2_Chd5 Expression in cytoplasm of cells.
Specimen 1B Rho: embryonic day 10.5 (more )
Note: Triple labeled: red - Chd5; green - Nes (assay MGI:5705562); blue - DAPI.
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 1B Rho merge , 1B Rho Chd5 Expression in ventral rhombencephalon.
Specimen 1B Mes: embryonic day 10.5 (more )
Note: Triple labeled: red - Chd5; green - Nes (assay MGI:5705562); blue - DAPI.
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 1B Mes Chd5 , 1B Mes merge Expression in posterior mesencephalon.
Specimen 1B Drg: embryonic day 10.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS17: dorsal root ganglion Present Regionally restricted 1B Drg Chd5 , 1B Drg merge Expression localized to cytoplasm of neurons.
Specimen 2A: embryonic day 13.5 (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS21: forebrain Present Widespread 2A merge , 2A Chd5
TS21: hindbrain Present Widespread 2A merge , 2A Chd5
TS21: midbrain Present Widespread 2A merge , 2A Chd5
Specimen 2B Rho: embryonic day 13.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS21: hindbrain Present Regionally restricted 2B Rho Chd5 , 2B Rho merge Expression predominantly in NeuN (Rbfox3)-positive neurons in the rhombencephalon.
Specimen 2B Mes: embryonic day 13.5 (more )
Note: TTriple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted 2B Mes Chd5 , 2B Mes merge Expression nestin-positive progenitor cells.
Specimen 2B Tel (lower middle): embryonic day 13.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 2B Tel (lower middle) Chd5 , 2B Tel (lower middle) merge Expression in cytoplasm of NeuN (Rbfox3)-positive differentiating neurons located in dorsal regions of telencephalon.
Specimen 2B Tel (lower): embryonic day 13.5 (more )
Note: Triple labeled: red - Chd5; green - Nes (assay MGI:5705562); blue - DAPI.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 2B Tel (lower) Chd5 , 2B Tel (lower) merge Expression not co-localized with nestin-postiive progeitors in ventral region of telencephalon.
Specimen S3A: embryonic day 13.5 (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS21: forebrain Present S3A merge , S3A Chd5
Specimen S3B: embryonic day 13.5 (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted S3B merge , S3B Chd5 Border of intense vs. less intense expression.
Specimen S3C: embryonic day 13.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS21: forebrain Present Regionally restricted S3C merge , S3C Chd5 Expression is nuclear in NeuN (Rbfox3)-positive differentiated neurons.
Specimen 3A Thal: embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS26: thalamus Present 3A Thal merge , 3A Thal Chd5
Specimen 3A Ctx: embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS26: cerebral cortex Present 3A Ctx Chd5 , 3A Ctx merge
Specimen 3A Hip: embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS26: hippocampus Present 3A Hip merge , 3A Hip Chd5
Specimen 3B Ctx (upper): embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS26: cerebral cortex Present Widespread 3B Ctx (upper) Chd5 , 3B Ctx (upper) merge Expression in all cortical layers.
Specimen 3B Hip: embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS26: hippocampus CA1 Present 3B Hip merge , 3B Hip Chd5
TS26: hippocampus CA2 Present 3B Hip merge , 3B Hip Chd5
TS26: hippocampus CA3 Present 3B Hip merge , 3B Hip Chd5
TS26: dentate gyrus Absent 3B Hip merge , 3B Hip Chd5
Specimen 3B Ctx (lower middle): embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - TuJ1 (Tubb3; assay MGI:5705570); blue - DAPI.
Structure Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted 3B Ctx (lower middle) merge , 3B Ctx (lower middle) Chd5 Expression in differentiating neurons.
Specimen 3B Ctx (lower): embryonic day 18.5 (more )
Note: Triple labeled: red - Chd5; green - TuJ1 (Tubb3; assay MGI:5705570); blue - DAPI.
Structure Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted 3B Ctx (lower) Chd5 , 3B Ctx (lower) merge Expression in differentiating neurons.
Specimen 4A: postnatal adult (more )
Note: Double labeled: red - Chd5; blue - DAPI.
Structure Level Pattern Image Note
TS28: brain Present Widespread 4A Chd5
Specimen 4B Ctx: postnatal adult (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS28: cerebral cortex Present Regionally restricted 4B Ctx Chd5 , 4B Ctx merge Expression in NeuN (Rbfox3)-positive neurons.
Specimen 4B Hip: postnatal adult (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS28: hippocampus Present Regionally restricted 4B Hip merge , 4B Hip Chd5 Expression in NeuN (Rbfox3)-positive neurons.
Specimen 4B Ob: postnatal adult (more )
Note: Triple labeled: red - Chd5; green - NeuN (Rbfox3; assay MGI:5705565); blue - DAPI.
Structure Level Pattern Image Note
TS28: main olfactory bulb Present Regionally restricted 4B Ob Chd5 , 4B Ob merge Expression in NeuN (Rbfox3)-positive neurons.
Specimen 4B Cb: postnatal adult (more )
Note: Triple labeled: red - Chd5; green - Calb1 (assay MGI:5705571); blue - DAPI.
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Strong 4B Cb Chd5 , 4B Cb merge Robust expression in Purkinje neurons.
TS28: cerebellum granule cell layer Present 4B Cb merge , 4B Cb Chd5 Lower levels of expression in molecular and granular layer neurons.
TS28: cerebellum molecular layer Present 4B Cb merge , 4B Cb Chd5 Lower levels of expression in molecular and granular layer neurons.
Specimen 5 Ctx (upper): postnatal (more )
Note: Triple labeled: red - Chd5; green - S100b (assay MGI:5705572); blue - DAPI.
Structure Level Pattern Image Note
TS28: cerebral cortex Present Regionally restricted 5_Ctx (upper) merge , 5_Ctx (upper) Chd5 No expression in astrocytes.
Specimen 5 Hip: postnatal (more )
Note: Triple labeled: red - Chd5; green - S100b (assay MGI:5705572); blue - DAPI.
Structure Level Pattern Image Note
TS28: hippocampus Present Regionally restricted 5_Hip Chd5 , 5_Hip merge No expression in astrocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory