Gene Expression Data
Assay Details
Assay
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Reference:
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J:193529
Aguado C, et al., Differential maturation of GIRK2-expressing neurons in the mouse cerebellum. J Chem Neuroanat. 2013 Jan;47:79-89
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5707612
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Gene symbol:
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Kcnj6
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Gene name:
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potassium inwardly-rectifying channel, subfamily J, member 6
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Antibody:
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Anti-Girk2
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Detection system:
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Secondary antibody
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Results
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Specimen
3A:
embryonic day 18.0
(more )
Note: Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
Specimen
3A:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebellum external granule cell layer |
Present |
Regionally restricted |
3A3
,
3A1
|
Expression was detected in the inner part of the external granule cell layer. The outer part of the external granule cell layer was negative.
|
TS26: cerebellum |
Present |
Regionally restricted |
3A1
,
3A3
|
Expression deeper in the cerebellar anlage and inner granule cell layer co-expressed Pax6. There was no co-expression with Pax6 in the rhombic lip.
|
Specimen
3B,D:
embryonic day 18.0
(more )
Note: Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
Specimen
3B,D:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebellum external granule cell layer |
Present |
Regionally restricted |
3B1
,
3B3
|
Expression was detected in the inner part of the external granule cell layer. The outer part of the external granule cell layer was negative.
|
TS26: cerebellum |
Present |
Regionally restricted |
3D3
,
3D1
,
3B3
,
3B1
|
Expression deeper in the cerebellar anlage and inner granule cell layer co-expressed Pax6.
|
Specimen
3C:
embryonic day 18.0
(more )
Note: Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
Specimen
3C:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Kcnj6; green - Pax6 (assay MGI:5707616).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebellum external granule cell layer |
Present |
Regionally restricted |
3C1
,
3C3
|
Expression was detected in the inner part of the external granule cell layer. The outer part of the external granule cell layer was negative.
|
TS26: cerebellum |
Present |
Regionally restricted |
3C3
,
3C1
|
Expression deeper in the cerebellar anlage and inner granule cell layer co-expressed Pax6. There was no co-expression with Pax6 in the rhombic lip.
|
Specimen
6A:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Kcnj6; blue - Nrgn (assay MGI:5707687); green - Gad1 (assay MGI:5707688).
|
Specimen
6B:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
Mutant Allele(s):
|
Gad1tm1.1Tama/Gad1+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Kcnj6; blue - Nrgn (assay MGI:5707687); green - Gad1 (assay MGI:5707688).
|
Specimen
6C:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
Mutant Allele(s):
|
Gad1tm1.1Tama/Gad1+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Kcnj6; blue - Grm2 (assay MGI:5707691) and Grm3 (assay MGI:5707694); green - Gad1 (assay MGI:5707688).
|
Specimen
6D:
(close )
Genetic Background:
|
involves: C57BL/6NCrlj * CBA/JNCrlj
|
Mutant Allele(s):
|
Gad1tm1.1Tama/Gad1+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Kcnj6; blue - Grm2 (assay MGI:5707691) and Grm3 (assay MGI:5707694); green - Gad1 (assay MGI:5707688).
|
|