Tgfb2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:11609 Pelton RW, et al., In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2. Development. 1990 Oct;110(2):609-20
Assay type:	RNA in situ
MGI Accession ID:	MGI:5758941
Gene symbol:	Tgfb2
Gene name:	transforming growth factor, beta 2

Probe:	Tgfb2 probe3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were reduced to an average size of 100-150 nucleotides by limited alkaline hydrolysis.

Results

3B/E ©

Specimen 3B/E: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: hindlimb skeleton	Very strong	Regionally restricted	3B/E	Expression is in the perichondrium of the cartilage, absent in mature cartilage.

4C ©

Specimen 4C: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: maxilla	Present	Regionally restricted	4C	Expression is in osteoblasts.

5B/E ©

Specimen 5B/E: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Weak	Regionally restricted	5B/E	Expression is in the non-condensed mesenchyme, absent in the condensed mesenchyme.
TS21: tooth epithelium	Absent		5B/E

5H/K ©

Specimen 5H/K: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Strong	Regionally restricted	5H/K	Expression is in the odontoblast layer and low expression in the pulp.
TS24: enamel organ	Absent		5H/K

7G/H ©

Specimen 7G/H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cochlea epithelium	Strong	Regionally restricted	7G/H	Expression is in the ciliated epithelia.

8C/D ©

Specimen 8C/D: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: vomeronasal organ sensory epithelium	Very strong		8C/D
TS21: nasal septum	Present	Regionally restricted	8C/D	Expression is in the perichondrium surrounding the cartilage.
TS21: vomeronasal organ mesenchyme	Absent		8C/D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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