Probe:	Tgfb1 probe9
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	2A	Strong expression in mesenchymal cells forming the vascular zone near the central artery of the maternal part of placenta.

Structure	Level	Pattern	Image	Note
TS17: chorion	Strong	Regionally restricted	2D	High levels of expression in a small number of cells that had the appearance of blood cells scattered throughout the connective tissue of the chorion.

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Very strong	Regionally restricted	2E	Extremely high level of expression in a small number of hematopoietic cells in the blood-forming island.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Scattered	3A
TS17: 1st branchial arch mandibular component	Strong		3A
TS17: 1st branchial arch maxillary component	Strong		3A
TS17: liver	Strong		3A
TS17: mesenchyme derived from neural crest	Strong	Regionally restricted	3A	Strong expression in single cells surrounding the somites.

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Strong	Regionally restricted	3E	Strong expression restricted to mesodermal hematopoietic precursor cells, single cells scattered throughout the endodermal strands of prehepatocytes.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong	Regionally restricted	3F/G	Strong expression in cells lining the nervous system.
TS17: nasal process mesenchyme	Present	Scattered	3F/G	Expression in a small number of cells scattered throughout the nasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present	Regionally restricted	4A	Expression in intersegmental cells of the early spinal column and in cells lining the cerebral ganglia.
TS20: spinal cord	Present	Regionally restricted	4A	Expression in intersegmental cells of the early spinal column and in cells lining the cerebral ganglia.
TS20: bronchus mesenchyme	Strong		4A

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	5A	Strong expression in narrow bands of osteoblasts adjacent to the vertebra and ribs.
TS22: vertebra	Strong	Regionally restricted	5A	Strong expression in narrow bands of osteoblasts adjacent to the vertebra and ribs.
TS22: cartilage condensation	Weak	Regionally restricted	5A	Weak expression in less differentiated elements of the developing axial skeleton, such as the sternum, and limb.
TS22: sternum	Weak	Regionally restricted	5A	Weak expression in less differentiated elements of the developing axial skeleton, such as the sternum, and limb.
TS22: lower jaw	Strong	Regionally restricted	5A	High levels of expression in ossifying tissues of the upper and lower jaw.
TS22: upper jaw	Strong	Regionally restricted	5A	High levels of expression in ossifying tissues of the upper and lower jaw.
TS22: bronchus mesenchyme	Strong		5A
TS22: gut mesenchyme	Strong	Regionally restricted	5A	Strong expression in mesodermal cell layers of submucosa.
TS22: gut epithelium	Absent		5A
TS22: mesenchyme of metanephros	Present	Regionally restricted	5A	Expression in surrounding stromal mesenchyme during formation of the metanephros.
TS22: vibrissa root sheath	Strong	Regionally restricted	5A	High levels of expresison in ectodermal epithelium of external root sheath.
TS22: vibrissa dermal component	Moderate	Regionally restricted	5A	Only moderate expression in the primordia of the dermal sheaths.

Structure	Level	Pattern	Image	Note
TS24: rib	Strong	Regionally restricted	6A	Strong expression in vertebra and ribs, both in periosteal layer and in ossification centers.
TS24: vertebra	Strong	Regionally restricted	6A	Strong expression in vertebra and ribs, both in periosteal layer and in ossification centers.
TS24: lower jaw skeleton	Strong	Regionally restricted	6A	High levels of expression in ossifying tissues of the upper and lower jaw.
TS24: upper jaw skeleton	Strong	Regionally restricted	6A	High levels of expression in ossifying tissues of the upper and lower jaw.
TS24: bronchus mesenchyme	Strong		6A
TS24: skin	Present	Regionally restricted	6A	Expression in loose mesenchyme underlying the epidermis.
TS24: dermis	Strong	Regionally restricted	6A	Within the dermis, high levels of expression in a small number of cells scattered between moderately or weakly labeled mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Widespread	7A	Expression in the majority of fetal liver cells. Weak expression in endodermal prehepatocytes.
TS20: hematopoietic system	Strong	Regionally restricted	7A	Very high levels of expression confined to megakaryocytes scattered throughout the fetal liver. Moderate levels of exprssion in other hematopoietic precursor cells.

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Present	Regionally restricted	7B	Expression in all cells of fetal thymus, but level of expression is variable among individual cells. Stronger expression in T-cell precursors than endodermal cell population.

Structure	Level	Pattern	Image	Note
TS24: upper jaw skeleton	Strong		8A	Strong expression in upper jaw bone tissues.
TS24: ethmoid bone	Weak	Regionally restricted	8A	Weak expression in perichondrial layer of ethmoid cartilages.

Structure	Level	Pattern	Image	Note
TS14: hepatic primordium	Absent

Structure	Level	Pattern	Image	Note
TS20: cochlea epithelium	Absent

Structure	Level	Pattern	Image	Note
TS22: hematopoietic system	Strong	Regionally restricted		Expression remained very strong in megakaryocytes, but decreased in other cells of the fetal liver
TS22: liver	Present	Regionally restricted		Expression remained very strong in megakaryocytes, but decreased in other cells of the fetal liver

Structure	Level	Pattern	Image	Note
TS24: hematopoietic system	Strong	Regionally restricted		Expression remained very strong in megakaryocytes, but decreased in other cells of the fetal liver
TS24: liver	Present	Regionally restricted		Expression remained very strong in megakaryocytes, but decreased in other cells of the fetal liver
TS24: cochlea epithelium	Absent
TS24: vibrissa inner root sheath	Present			Expression in both inner and outer root sheath of whisker follicles.
TS24: vibrissa outer root sheath	Present			Expression in both inner and outer root sheath of whisker follicles.
TS24: vibrissa root sheath matrix	Weak	Regionally restricted		Low levels of expression in connective tissue sheath of the hair follicle.

Structure	Level	Pattern	Image	Note
TS26: hematopoietic system	Present	Regionally restricted		Expression detectable in megakaryocytes.
TS26: liver	Present	Regionally restricted		Expression detectable in megakaryocytes.
TS26: olfactory epithelium	Absent