Gene Expression Data
Assay Details
Assay
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Reference:
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J:31180
Schmid P, et al., Differential expression of TGF beta 1, beta 2 and beta 3 genes during mouse embryogenesis. Development. 1991 Jan;111(1):117-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5774642
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Gene symbol:
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Tgfb2
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Gene name:
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transforming growth factor, beta 2
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Probe:
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Tgfb2 probe8
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2B maternal:
embryonic day 10.5
(more )
Specimen
2B maternal:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Absent |
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2B
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Only non-specific background signals are visible.
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Specimen
3B:
embryonic day 10.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: embryo |
Absent |
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3B
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Authors state that, compared to panel A, only non-specific background signals are visible.
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Specimen
4B:
embryonic day 12.5
(more )
Specimen
4B:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: vertebral cartilage condensation |
Strong |
Regionally restricted |
4B
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High levels of expression in primordia of vertebral column.
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TS20: sclerotome |
Very strong |
Regionally restricted |
4B
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Strongest expression in caudal sclerotomic halves. High levels of expression only in the thoracic sclerotomes.
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TS20: lung epithelium |
Present |
Regionally restricted |
4B
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Expression in endodermal bronchiolar epithelia.
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TS20: gut mesentery |
Present |
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4B
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Expression in single cell layer forming mesodermal mesentery.
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Specimen
5B:
embryonic day 14.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7C:
embryonic day 14.5
(more )
Specimen
7C:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8B:
embryonic day 16.5
(more )
Specimen
8B:
(close )
Genetic Background:
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Rb(4.15)4Rma
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: upper jaw skeleton |
Absent |
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8B
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No expression in bone tissue of upper jaw.
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TS24: ethmoid bone |
Weak |
Regionally restricted |
8B
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Low levels of expression in perichondrium of ethmoid cartilages.
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TS24: olfactory epithelium |
Present |
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8B
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Expression in olfactory epithelium of the nasal chamber.
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TS24: mandible |
Present |
Regionally restricted |
8B
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Expression in nasal and mandibular mesenchyme that forms the soft tissue components of the face.
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TS24: nasal mesenchyme |
Present |
Regionally restricted |
8B
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Expression in nasal and mandibular mesenchyme that forms the soft tissue components of the face.
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Specimen
day 10.5 not shown:
embryonic day 10.5
(more )
Specimen
day 10.5 not shown:
(close )
Genetic Background:
|
Rb(4.15)4Rma
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
day 12.5 not shown:
embryonic day 12.5
(more )
Specimen
day 12.5 not shown:
(close )
Genetic Background:
|
Rb(4.15)4Rma
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
day 16.5 not shown:
embryonic day 16.5
(more )
Specimen
day 16.5 not shown:
(close )
Genetic Background:
|
Rb(4.15)4Rma
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
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