Gene Expression Data
Assay Details
Assay
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Reference:
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J:169043
Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5790062
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Gene symbol:
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Dll1
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Gene name:
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delta like canonical Notch ligand 1
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Antibody:
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PGPM-1F9
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Detection system:
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Secondary antibody
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Results
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Specimen
1H:
embryonic day 9.5
(more )
Note: Freshly dissected unfixed non-permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Specimen
1H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Freshly dissected unfixed non-permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1H Dll1
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Expression is in the presomitic mesoderm in this non-permabilized specimen.
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Specimen
1I:
embryonic day 9.5
(more )
Note: Freshly dissected unfixed permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Specimen
1I:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Freshly dissected unfixed permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
1I Dll1
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Expression is in the presomitic mesoderm in this permabilized specimen.
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Specimen
3H:
embryonic day 9.5
(more )
Note: (I) is a higher magnification of (H), inset is a higher magnification of the boxed region. Double labeled: green - Dll1; red - Notch1 (assay MGI:5790067).
Specimen
3H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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(I) is a higher magnification of (H), inset is a higher magnification of the boxed region. Double labeled: green - Dll1; red - Notch1 (assay MGI:5790067).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
3I merge
,
3I Dll1
,
3H merge
,
3H Dll1
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Expression is in the rostral presomitic mesoderm.
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Specimen
3J:
embryonic day 9.5
(more )
Note: Triple labeled: green - Dll1; red - Notch1 (assay MGI:5790067); blue - Dll3 (assay MGI:5790068).
Specimen
3J:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: green - Dll1; red - Notch1 (assay MGI:5790067); blue - Dll3 (assay MGI:5790068).
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Specimen
4E:
embryonic day 9.5
(more )
Note: (G and H) are higher magnifications of (E). Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Specimen
4E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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(G and H) are higher magnifications of (E). Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4E merge
,
4G Dll1
,
4G merge
,
4H
,
4E Dll1
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Expression is in the rostral presomitic mesoderm and is strongest at its rostral limit.
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TS15: somite |
Absent |
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4G Dll1
,
4H
,
4E merge
,
4G merge
,
4E Dll1
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Specimen
4I:
(close )
Genetic Background:
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involves: 129P2/Ola
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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(J) is a higher magnification of (I). Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4J Dll1
,
4I Dll1
,
4I merge
,
4J merge
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Expression is in the rostral presomitic mesoderm.
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Specimen
S3a:
embryonic day 9.5
(more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Specimen
S3a:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Note:
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Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3a merge
,
S3a Dll1
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Expression is in the rostral presomitic mesoderm.
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Specimen
S3b:
embryonic day 9.5
(more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Specimen
S3b:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3b merge
,
S3b Dll1
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Expression is in the rostral presomitic mesoderm.
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Specimen
S3c:
embryonic day 11.5
(more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Specimen
S3c:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3c merge
,
S3c Dll1
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Expression is in the rostral presomitic mesoderm.
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Specimen
S3d:
embryonic day 11.5
(more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Specimen
S3d:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Note:
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Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3d Dll1
,
S3d merge
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Expression is in the rostral presomitic mesoderm.
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