Gene Expression Data
Assay Details
Assay
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Reference:
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J:169043
Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5790075
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Gene symbol:
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Notch1
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Gene name:
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notch 1
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Results
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Specimen
3K:
embryonic day 9.5
(more )
Note: Inset is a higher magnification of the boxed region. Triple labeled: blue - cleaved Notch1; green - Notch1 (assay MGI:5790067); red - Dll3 (assay MGI:5790068).
Specimen
3K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Inset is a higher magnification of the boxed region. Triple labeled: blue - cleaved Notch1; green - Notch1 (assay MGI:5790067); red - Dll3 (assay MGI:5790068).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
3K cleaved Notch1
,
3K merge
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Expression is in the rostral presomitic mesoderm.
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Specimen
4A:
embryonic day 9.5
(more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Specimen
4A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
|
Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4A
|
Expression is in thin stripes of cells in the rostral presomitic mesoderm.
|
Specimen
4B:
embryonic day 9.5
(more )
Note: Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
Specimen
4B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
|
Type:
|
whole mount
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Note:
|
Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4B
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Expression is in thin stripes of cells in the rostral presomitic mesoderm.
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Specimen
4C:
(close )
Genetic Background:
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involves: 129P2/Ola
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Note:
|
Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4C
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In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
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Specimen
4D:
(close )
Genetic Background:
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involves: 129P2/Ola
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Note:
|
Double labeled: red - cleaved Notch1; green - Notch1 (assay MGI:5790067).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4D
|
In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
|
Specimen
4E:
embryonic day 9.5
(more )
Note: (G and H) are higher magnifications of (E). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
4E:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
|
Note:
|
(G and H) are higher magnifications of (E). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4E merge
,
4E cleaved Notch1
,
4G merge
,
4H
,
4G cleaved Notch1
|
Expression is in the rostral presomitic mesoderm.
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Specimen
4I:
embryonic day 9.5; Dll3tm1Rbe/ Dll3tm1Rbe
(more )
Note: (J) is a higher magnification of (I). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
4I:
(close )
Genetic Background:
|
involves: 129P2/Ola
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Mutant Allele(s):
|
Dll3tm1Rbe/Dll3tm1Rbe
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Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Note:
|
(J) is a higher magnification of (I). Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
4J merge
,
4J cleaved Notch1
,
4I merge
,
4I cleaved Notch1
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In this mutant an expression domain in the rostral presomitic mesoderm is missing and there is one broad rostral band of expression.
|
Specimen
S3a:
embryonic day 9.5
(more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
S3a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3a merge
,
S3a cleaved Notch1
|
Expression is in thin stripes of cells in the rostral presomitic mesoderm.
|
Specimen
S3b:
embryonic day 9.5
(more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
S3b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Note:
|
Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3b merge
,
S3b cleaved Notch1
|
Expression is in thin stripes of cells in the rostral presomitic mesoderm.
|
Specimen
S3c:
embryonic day 11.5
(more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
S3c:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
|
Type:
|
whole mount
|
Note:
|
Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3c merge
,
S3c cleaved Notch1
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Expression is in thin stripes of cells in the rostral presomitic mesoderm.
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Specimen
S3d:
embryonic day 11.5
(more )
Note: Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
Specimen
S3d:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
|
Note:
|
Triple labeled: red - cleaved Notch1; green - Dll3 (assay MGI:5790068); blue - Dll1 (assay MGI:5790062).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail unsegmented mesenchyme |
Present |
Regionally restricted |
S3d merge
,
S3d cleaved Notch1
|
Expression is in thin stripes of cells in the rostral presomitic mesoderm.
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