Gene Expression Data
Assay Details
Assay
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Reference:
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J:135516
Wansbury O, et al., Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J Invest Dermatol. 2008 Apr;128(4):1009-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5800274
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Gene symbol:
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Nrg3
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Gene name:
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neuregulin 3
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Antibody:
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Nrg3 EGF-like domain antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative was either: 4% paraformaldehyde, formalin, or Carnoy's fixative. Three different polyclonal antibodies may have been used: Nrg3 (P-18) antibody (MGI:5800276), Nrg3 (D-19) antibody (MGI:5800277, or Nrg3 antibody (MGI:5800291).
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Results
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Specimen
8f:
embryonic day 11.5
(more )
Specimen
8f:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8g:
embryonic day 12.5
(more )
Specimen
8g:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: mammary bud |
Present |
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8g
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TS20: epidermis |
Present |
Regionally restricted |
8g
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Expression is in the epithelium adjacent to the mammary bud.
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Specimen
8h:
embryonic day 13.5
(more )
Specimen
8h:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8i:
embryonic day 14.5
(more )
Specimen
8i:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: mammary bud |
Weak |
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8i
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Expression is higher in the neck of the bud.
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TS22: epidermis |
Present |
Regionally restricted |
8i
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Expression is in the epithelium adjacent to the mammary bud.
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TS22: connective tissue |
Present |
Regionally restricted |
8i
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Expression is at the site of the future mammary fat pad.
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Specimen
S2:
embryonic day 12.5
(more )
Specimen
S2:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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