Gene Expression Data
Assay Details
Assay
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Reference:
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J:96871
Chi H, et al., Loss of mitogen-activated protein kinase kinase kinase 4 (MEKK4) results in enhanced apoptosis and defective neural tube development. Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3846-51
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5804189
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Gene symbol:
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Mapk9
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Gene name:
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mitogen-activated protein kinase 9
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Antibody:
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Anti-Phospho-JNK
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The antibody used in this assay recognized the phosphorylated form of the protein.
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Results
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Specimen
7A Top MEKK4+/+:
embryonic day 9.5
(more )
Specimen
7A Top MEKK4+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A Top MEKK4-/-:
(close )
Genetic Background:
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B6.129S6-Map3k4tm1Flv
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Strong |
Regionally restricted |
7A HB Mekk4-/-
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Expression was stronger toward the edges of the hindbrain neural folds. There was no significant difference between +/+ and -/- embryos.
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Specimen
7A Bottom MEKK4+/+:
embryonic day 9.5
(more )
Specimen
7A Bottom MEKK4+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A Bottom MEKK4-/-:
(close )
Genetic Background:
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B6.129S6-Map3k4tm1Flv
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Mutant Allele(s):
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Map3k4tm1Flv/Map3k4tm1Flv
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future forebrain |
Strong |
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7A FB Mekk4-/-
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Expression was strong in the forebrain neuroepithelium. There was no significant difference between +/+ and -/- embryos.
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