Reporter:	GFP
Antibody:	anti-GFP (Aves)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS28: adrenal gland	Present		1B

Structure	Level	Pattern	Image	Note
TS17: future neurohypophysis	Present	Regionally restricted	2A	Expressed in the median eminence.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2C	Expressed in fibers extending into the medial forebrain bundle and traveling toward the epithalamus.

Structure	Level	Pattern	Image	Note
TS20: median eminence	Present		3A

Structure	Level	Pattern	Image	Note
TS20: median eminence	Present		3C
TS20: forebrain	Present	Regionally restricted	3C	Expressed in fibers extending into the medial forebrain bundle, and the caudal, lateral postoptic area. Positive fibers travel medial to the optic tract and lateral to the medial forebrain bundle, following a path similar to the presumptive ventral supraoptic commissure.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	3E	Expressed in the presumptive ventromedial nucleus of the hypothalamus and fibers, with positive neurons occupying the lateral half of the hypothalamus and extending to the edge of the developing brain.

Structure	Level	Pattern	Image	Note
TS22: median eminence	Present		4A
TS22: diencephalon	Present	Regionally restricted	4A	Expressed in fibers in the median eminence and extending into the ventral supraoptic commissure.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4C	Expressed in neurons in the ventromedial nucleus of the hypothalamus.
TS22: diencephalon	Present	Regionally restricted	4C	Expressed in fibers extending dorsally along the ventral supraoptic commissure. A second, scattered group of fibers traverse through the medial forebrain bundle, ascending laterally and dorsolaterally in a fan-like pattern.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4E	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
TS22: diencephalon	Present	Regionally restricted	4E	Expressed in fibers traveling in the medial forebrain bundle angle toward and join the ventral supraoptic commissure at the level of the external medullary lamina. Most of these fibers travel along the lateral wall of the developing brain and branch toward the epithalamus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4G	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.

Structure	Level	Pattern	Image	Note
TS25: lateral preoptic area	Absent		6A
TS25: suprachiasmatic nucleus	Absent		6A
TS25: hypothalamus	Present	Regionally restricted	6A	Expressed in fibers surrounding, but sparing, the suprachiasmatic nucleus. Hypothalamic paraventricular nucleus and the anterior hypothalamus also receive fiber projections.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6C	Expressed in neurons in the ventromedial nucleus, ventral supraoptic commissure and retrochiasmatic area of the hypothalamus.
TS25: forebrain	Present	Regionally restricted	6C	Expressed in ascending fiber tract traveling through the medial aspect of the hypothalamus and reaches the medial basal forebrain.
TS25: lateral preoptic area	Absent		6C
TS25: suprachiasmatic nucleus	Absent		6C

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6E	Expressed in neurons in the ventromedial nucleus (with an absence of positive cells in the most lateral aspect) and ventral supraoptic commissure of the hypothalamus.
TS25: forebrain	Present	Regionally restricted	6E	Expressed in fibers emerging from the dorsallateral aspect of the nucleus and travel parallel to the ventral supraoptic commissure at the lateral aspect of the medial lemniscus.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6G	Expressed in neurons in the ventromedial nucleus (with an absence of positive cells in the most lateral aspect) and ventral supraoptic commissure of the hypothalamus.
TS25: forebrain	Present	Regionally restricted	6G	Expressed in fibers emerging from the dorsallateral aspect of the nucleus and travel parallel to the ventral supraoptic commissure at the lateral aspect of the medial lemniscus.

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present	Regionally restricted	6I	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
TS25: forebrain	Present	Regionally restricted	6I	Expressed in fibers originates from the posterior aspect of the ventromedial nucleus and follows the periventricular system extending along the midline of the brain to traverse through the dorsomedial nucleus and posterior hypothalamus to reach the periaqueductal gray region.

Structure	Level	Pattern	Image	Note
TS25: periaqueductal grey	Present		6K
TS25: superior colliculus	Present	Regionally restricted	6K	Expressed in the deeper layer.

Structure	Level	Pattern	Image	Note
TS27: hypothalamus periventricular zone	Present		7A
TS27: superior colliculus	Present		7A
TS27: ventromedial hypothalamic nucleus	Present	Regionally restricted	7A	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.
TS27: supraoptic nucleus	Present	Regionally restricted	7A	Expressed in ventral supraoptic commissure.

Structure	Level	Pattern	Image	Note
TS27: supraoptic nucleus	Present	Regionally restricted	7C	Expressed in ventral supraoptic commissure.
TS27: medial geniculate nucleus	Present		7C
TS27: periaqueductal grey	Present		7C
TS27: midbrain	Present	Regionally restricted	7C	Expressed in fibers in the ventral supraoptic commissure along the internal capsule, across the medial geniculate nucleus, and into the periaqueductal grey.

Structure	Level	Pattern	Image	Note
TS27: periaqueductal grey	Present		7E

Structure	Level	Pattern	Image	Note
TS27: ventromedial hypothalamic nucleus	Present	Regionally restricted	7G	Expressed in neurons in the ventromedial nucleus of the hypothalamus, with an absence of positive cells in the most lateral aspect.

Structure	Level	Pattern	Image	Note
TS28: pituitary gland	Present
TS28: testis	Present