Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:119664
Houde C, et al., Hippi is essential for node cilia assembly and Sonic hedgehog signaling. Dev Biol. 2006 Dec 15;300(2):523-33
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5807056
|
Gene symbol:
|
Nodal
|
Gene name:
|
nodal growth differentiation factor
|
Probe:
|
Nodal probe21
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
4A:
embryonic day 8.0
(more )
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S1/SvImJ * C57BL/6J
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4B:
(close )
Genetic Background:
|
involves: 129S1/SvImJ * C57BL/6J
|
Mutant Allele(s):
|
Ift57tm1Dwni/Ift57+
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Strong |
Regionally restricted |
4B
|
Expression is asymmetrical. Strong expression in the left lateral plate mesoderm. Authors state that heterozygotes always stain as the wildtype embryos.
|
TS12: node |
Present |
|
4B
|
|
Specimen
4C:
(close )
Genetic Background:
|
involves: 129S1/SvImJ * C57BL/6J
|
Mutant Allele(s):
|
Ift57tm1Dwni/Ift57tm1Dwni
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: lateral plate mesoderm |
Strong |
Regionally restricted |
4C
|
Expression is detected bilaterally in both the left and right lateral plate mesoderm.
|
TS12: node |
Weak |
|
4C
|
Downregulation of expression around the node region compared to wildtype and heterozygous embryos.
|
|