Probe:	Meis2b cDNA
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present			Expression mostly in neural and non-neural ectoderm.
TS11: non-neural ectoderm	Present			Expression mostly in neural and non-neural ectoderm.

Structure	Level	Pattern	Image	Note
TS12: neural tube	Present	Widespread	5A	Expression distributed throughout the neural tube.
TS12: embryo mesoderm	Present	Regionally restricted	5A	Authors report expression in various mesodermal regions.
TS12: somite	Strong		5A	Stronger expression in somitic and presomitic mesoderm.
TS12: trunk unsegmented mesenchyme	Strong		5A	Stronger expression in somitic and presomitic mesoderm.
TS12: lateral plate mesoderm	Present		5A

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	5B	Sharply defined expression domain in midbrain.
TS15: telencephalon	Present		5B	Clear expression in telencephalic neurectoderm.
TS15: optic stalk	Present		5B
TS15: heart	Strong	Regionally restricted	5B	Strong expression in dorsal regions of the heart.
TS15: trunk mesenchyme	Strong	Regionally restricted	5B	Strong expression in trunk mesenchyme at level of forelimb buds.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted		Authors report expression in specific areas of brain.
TS17: hindlimb bud	Present	Regionally restricted		Expression restricted to proximal regions of hindlimb buds.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5C	Expression in telencephalic vesicles and ganglionic eminence.
TS19: diencephalon	Present	Regionally restricted	5C	Expression in part of dorsal thalamus.
TS19: midbrain	Present	Regionally restricted	5C	Expression restricted to tectum and pyramidal tract.
TS19: hindbrain	Present	Regionally restricted	5C	No expression in a region between the pons and rhombic isthmus.
TS19: cranial ganglion	Present		5C
TS19: dorsal root ganglion	Present		5C
TS19: 1st branchial arch maxillary component	Present		5C	Authors report discrete expression in maxillary region.
TS19: 1st branchial arch	Present	Regionally restricted	5C	Expression in maxillo-mandibular cleft epithelium.
TS19: fronto-nasal process mesenchyme	Absent		5C
TS19: mandibular process mesenchyme	Absent		5C
TS19: inner ear epithelium	Present		5C
TS19: nasal epithelium	Present		5C
TS19: oral epithelium	Present		5C
TS19: liver	Absent		5C
TS19: myotome	Present	Regionally restricted	5C	Expression persisted in myotomal derivatives.
TS19: sclerotome	Absent		5C

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	5D	Expression in telencephalic vesicles.
TS21: ganglionic eminence	Present		5D
TS21: diencephalon	Present	Regionally restricted	5D	Expression in part of dorsal thalamus.
TS21: maxilla	Present		5D	Expression persisted in derivatives of maxillary process.
TS21: ear epithelium	Present		5D
TS21: nasal cavity epithelium	Present		5D
TS21: oral epithelium	Present		5D
TS21: mandible	Absent		5D	No expression in distal mesenchyme of tongue, mandible, and nasal regions.
TS21: nasal mesenchyme	Absent		5D	No expression in distal mesenchyme of tongue, mandible, and nasal regions.
TS21: tongue mesenchyme	Absent		5D	No expression in distal mesenchyme of tongue, mandible, and nasal regions.
TS21: epidermis	Present		5D
TS21: vibrissa follicle	Present		5D
TS21: gut mesenchyme	Strong		5D
TS21: mesenchyme of metanephros	Strong		5D
TS21: stomach mesenchyme	Strong		5D
TS21: stomach epithelium	Present	Regionally restricted	5D	Specific expression in some regions of stomach glandular epithelium.
TS21: heart	Weak		5D
TS21: lung	Weak		5D
TS21: gonad	Absent		5D
TS21: liver	Absent		5D

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	6A	Expression distributed throughout the spinal cord. Stronger expression in ventral horns.
TS15: heart	Strong	Regionally restricted	6A	Strong expression in dorsal regions of the heart.
TS15: trunk mesenchyme	Strong	Regionally restricted	6A	Strong expression in trunk mesenchyme at level of forelimb buds.
TS15: forelimb bud mesenchyme	Present	Regionally restricted	6A	Expression extends in forelimb buds, gradually decreasing towards distal regions.
TS15: gut mesenchyme	Present		6A
TS15: gut endoderm	Absent		6A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	6B	Expression distributed throughout the spinal cord. Stronger expression in ventral horns.
TS17: heart	Strong	Regionally restricted	6B	Strong expression in dorsal regions of the heart.
TS17: trunk mesenchyme	Strong	Regionally restricted	6B	Strong expression in trunk mesenchyme at level of forelimb buds.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	6B	Expression restricted to proximal regions of forelimb buds.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6C	Expression in ventral horns.
TS19: neural tube mantle layer	Present	Regionally restricted	6C	Expression in more dorsal regions of mantle layer of spinal cord.
TS19: neural tube floor plate	Absent		6C
TS19: dorsal root ganglion	Present		6C
TS19: trunk myotome	Present		6C	Expression persisted in myotomal derivatives.
TS19: trunk sclerotome	Absent		6C

Structure	Level	Pattern	Image	Note
TS19: stomach epithelium	Present	Regionally restricted	7A	Specific expression in some regions of stomach glandular epithelium.
TS19: forelimb bud mesenchyme	Present	Regionally restricted	7A	Expression restricted to very proximal limb mesenchyme and to a central cell condensation.
TS19: forelimb bud ectoderm	Present	Regionally restricted	7A	Expression also detected in surface ectoderm of limbs.

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Strong		7B
TS21: mesenchyme of metanephros	Strong		7B
TS21: stomach mesenchyme	Strong		7B
TS21: gut epithelium	Absent		7B
TS21: hindlimb	Present	Regionally restricted	7B	Expression throughout developing tissues of proximal limb regions, except for differentiating cartilage and bone.
TS21: hindlimb cartilage condensation	Absent		7B	No expression in differentiating cartilage and bone.
TS21: foot epithelium	Present	Regionally restricted	7B	No expression in distal regions (footplates) except in surface ectoderm.
TS21: hindlimb interdigital region epithelium	Strong		7B	In surface ectoderm, higher expression in interdigital regions.
TS21: hindlimb interdigital region mesenchyme	Weak		7B	Weak expression in interdigital mesenchymal cells.