Probe:	Calml4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	2A Calml4 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2A Calml4 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Calml4 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Calml4 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Moderate	Regionally restricted	Calml4_b52_E10.5a_RF_31OCT13	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Moderate	Regionally restricted	Calml4_b52_E10.5b_RF_31OCT13	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Calml4_b52_E11.5a_RF_8NOV13	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS17: ear	Weak		Calml4_b52_E10.5a_31OCT13
TS17: somite	Weak		Calml4_b52_E10.5a_31OCT13

Structure	Level	Pattern	Image	Note
TS17: ear	Weak		Calml4_b52_E10.5b_31OCT13
TS17: somite	Weak		Calml4_b52_E10.5b_31OCT13

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Calml4_b52_E11.5a_8NOV13	Staining is similar to that observed at E10.5. At that time it was seen in the ear and somites.