Hand2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226028 Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
Assay type:	RNA in situ
MGI Accession ID:	MGI:5823629
Gene symbol:	Hand2
Gene name:	heart and neural crest derivatives expressed 2

Probe:	Hand2 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen S1A - Hand2 - E10.5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Hand2 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Specimen S1A - Hand2 - E11.5: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Hand2 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Specimen Hand2_E10.5a_RF_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Hand2_E10.5a_RF_JL	Staining is observed in the posterior limb bud.

Specimen Hand2_E10.5b_RF_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Hand2_E10.5b_RF_JL	Staining is observed in the posterior limb bud.

Specimen Hand2_E11.5a_RFJL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Hand2_E11.5a_RFJL	Domains of staining are similar to those seen at E10.5.

Specimen Hand2_E10.5a_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: heart	Weak		Hand2_E10.5a_JL
TS17: branchial arch	Strong		Hand2_E10.5a_JL

Specimen Hand2_E10.5b_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: heart	Weak		Hand2_E10.5b_JL
TS17: branchial arch	Strong		Hand2_E10.5b_JL

Specimen Hand2_E11.5a_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Hand2_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the heart and branchial arch.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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