Probe:	Hhip cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Hhip - E10.5	This gene exhibits category 2 expression: staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Hhip - E11.5	This gene exhibits category 2 expression: staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Moderate	Regionally restricted	Hhip_b4_E10.5a_RF_3JULY13_JL	Staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Hhip_b4_E11.5a_RF_8NOV13	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Hhip_b4_E11.5a_RH_8NOV13

Structure	Level	Pattern	Image	Note
TS17: somite	Strong		Hhip_b4_E10.5a_3JULY13_JL
TS17: branchial arch	Weak		Hhip_b4_E10.5a_3JULY13_JL
TS17: central nervous system	Weak		Hhip_b4_E10.5a_3JULY13_JL

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Hhip_b4_E11.5a_8NOV13	Staining is similar to that observed at E10.5. At that time it was seen in the somites, branchial arch and central nervous system.