Msi2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226028 Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
Assay type:	RNA in situ
MGI Accession ID:	MGI:5823668
Gene symbol:	Msi2
Gene name:	musashi RNA-binding protein 2

Probe:	Msi2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1C Msi2 ©

Specimen 1C - Msi2: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	1C Msi2	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

S1A Msi2 - E10.5 ©

Specimen S1A - Msi2 - E10.5: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Msi2 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

S1A Msi2 - E11.5 ©

Specimen S1A - Msi2 - E11.5: embryonic day 11.5 (more

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Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Msi2 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Msi2_b10_E10.5a_RF_3JUNE13 ©

Specimen Msi2_b10_E10.5a_RF_3JUNE13: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Msi2_b10_E10.5a_RF_3JUNE13	Staining is observed in the posterior-distal limb bud.

Msi2_b10_E10.5b_RF_3JUNE13 ©

Specimen Msi2_b10_E10.5b_RF_3JUNE13: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Msi2_b10_E10.5b_RF_3JUNE13	Staining is observed in the posterior-distal limb bud.

Msi2_b10_E10.5c_RF_JL-2 ©

Specimen Msi2_b10_E10.5c_RF_JL-2: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Msi2_b10_E10.5c_RF_JL-2	Staining is observed in the posterior-distal limb bud.

Msi2_b10_E11.5b_RF_JL ©

Specimen Msi2_b10_E11.5b_RF_JL: embryonic day 11.5 (more

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Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Msi2_b10_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Msi2_b10_E11.5c_HLR_JL ©

Specimen Msi2_b10_E11.5c_HLR_JL: embryonic day 11.5 (more

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Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Msi2_b10_E11.5c_HLR_JL

Specimen Msi2_b10_E10.5a_3JUNE13: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: branchial arch	Moderate		Msi2_b10_E10.5a_3JUNE13

Specimen Msi2_b10_E10.5b_3JUNE13: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Moderate		Msi2_b10_E10.5b_3JUNE13

Specimen Msi2_b10_E10.5c_JL: embryonic day 10.5 (more

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Structure	Level	Pattern	Image	Note
TS17: branchial arch	Moderate		Msi2_b10_E10.5c_JL

Specimen Msi2_b10_E11.5b_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Msi2_b10_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the branchial arch.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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