Probe:	Ptch1 probe15
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Ptch1 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Ptch1 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Ptch1_E10.5a_RF_JL	Staining is observed in the posterior limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Ptch1_E10.5b_RF_JL	Staining is observed in the posterior limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Ptch1_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS17: somite	Weak		Ptch1_E10.5a_JL
TS17: branchial arch	Moderate		Ptch1_E10.5a_JL
TS17: central nervous system	Strong		Ptch1_E10.5a_JL

Structure	Level	Pattern	Image	Note
TS17: somite	Weak		Ptch1_E10.5b_JL
TS17: branchial arch	Moderate		Ptch1_E10.5b_JL
TS17: central nervous system	Strong		Ptch1_E10.5b_JL

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Ptch1_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the somites, branchial arch and central nervous system.