Probe:	Ralgps2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Ralgps2 - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Ralgps2 - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak	Regionally restricted	Ralgps2_b23_E10.5b_RF_JL	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak	Regionally restricted	Ralgps2_b23_E10.5c_RF_3JULY13_JL	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak	Regionally restricted	Ralgps2_b23_E10.5d_RF_3JUNE13	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Ralgps2_b23_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Ralgps2_b23_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		Ralgps2_b23_E10.5a_RHL_JL

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Ralgps2_b23_E11.5b_RHL_JL

Structure	Level	Pattern	Image	Note
TS17: heart	Moderate		Ralgps2_b23_E10.5a_JL
TS17: somite	Moderate		Ralgps2_b23_E10.5a_JL
TS17: branchial arch	Moderate		Ralgps2_b23_E10.5a_JL

Structure	Level	Pattern	Image	Note
TS17: heart	Moderate		Ralgps2_b23_E10.5b_JL
TS17: somite	Moderate		Ralgps2_b23_E10.5b_JL
TS17: branchial arch	Moderate		Ralgps2_b23_E10.5b_JL

Structure	Level	Pattern	Image	Note
TS17: heart	Moderate		Ralgps2_b23_E10.5c_3JULY13_JL
TS17: somite	Moderate		Ralgps2_b23_E10.5c_3JULY13_JL
TS17: branchial arch	Moderate		Ralgps2_b23_E10.5c_3JULY13_JL

Structure	Level	Pattern	Image	Note
TS17: heart	Moderate		Ralgps2_b23_E10.5d_3JUNE13
TS17: somite	Moderate		Ralgps2_b23_E10.5d_3JUNE13
TS17: branchial arch	Moderate		Ralgps2_b23_E10.5d_3JUNE13

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Ralgps2_b23_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the heart, somites and branchial arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Ralgps2_b23_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the heart, somites and branchial arch.