Probe:	Rasgef1b cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	2A Rasgef1b - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2A Rasgef1b - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Rasgef1b - E10.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Rasgef1b - E11.5	This gene exhibits category 1 expression: staining is observed in the posterior/posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Rasgef1b_b9_E10.5a_RF_JL	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Rasgef1b_b9_E10.5b_RF_JL	Staining is observed in the posterior-distal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rasgef1b_b9_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rasgef1b_b9_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rasgef1b_b9_E11.5c_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Rasgef1b_b9_E11.5a_RHL_JL

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Rasgef1b_b9_E11.5b_HLR_JL

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Moderate		Rasgef1b_b9_E10.5a_JL

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Moderate		Rasgef1b_b9_E10.5b_JL

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rasgef1b_b9_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the branchial arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rasgef1b_b9_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the branchial arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rasgef1b_b9_E11.5c_JL	Staining is similar to that observed at E10.5. At that time it was seen in the branchial arch.