Probe:	Rspo3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Rspo3 - E10.5	This gene exhibits category 2 expression: staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Rspo3 - E11.5	This gene exhibits category 2 expression: staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Rspo3_b71_E10.5a_RF_JL	Staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Rspo3_b71_E10.5b_RF_JL	Staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Rspo3_b71_E10.5c_RF_3JUNE2013	Staining is observed in the central limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rspo3_b71_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rspo3_b71_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Rspo3_b71_E11.5c_RF_8NOV13	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		Rspo3_b71_E11.5a_RHL_JL

Structure	Level	Pattern	Image	Note
TS17: ear	Moderate		Rspo3_b71_E10.5a_JL
TS17: heart	Strong		Rspo3_b71_E10.5a_JL
TS17: somite	Moderate		Rspo3_b71_E10.5a_JL
TS17: branchial arch	Weak		Rspo3_b71_E10.5a_JL

Structure	Level	Pattern	Image	Note
TS17: ear	Moderate		Rspo3_b71_E10.5b_JL
TS17: heart	Strong		Rspo3_b71_E10.5b_JL
TS17: somite	Moderate		Rspo3_b71_E10.5b_JL
TS17: branchial arch	Weak		Rspo3_b71_E10.5b_JL

Structure	Level	Pattern	Image	Note
TS17: ear	Moderate		Rspo3_b71_E10.5c_3JUNE2013
TS17: heart	Strong		Rspo3_b71_E10.5c_3JUNE2013
TS17: somite	Moderate		Rspo3_b71_E10.5c_3JUNE2013
TS17: branchial arch	Weak		Rspo3_b71_E10.5c_3JUNE2013

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rspo3_b71_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the ear, heart, somites and branchial arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rspo3_b71_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the ear, heart, somites and branchial arch.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Rspo3_b71_E11.5c_8NOV13	Staining is similar to that observed at E10.5. At that time it was seen in the ear, heart, somites and branchial arch.