Gene Expression Data
Assay Details
Assay
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Reference:
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J:159506
Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5827739
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Gene symbol:
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Krt6
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Gene name:
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keratin 6
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Antibody:
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anti-K6
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1C:
embryonic day 16.5
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
1C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: mammary gland primordium |
Strong |
Regionally restricted |
1C
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Strong expression in nipple sheath. Scattered positive cells also in nipple skin between the sheath.
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TS24: mammary sprout |
Strong |
Regionally restricted |
1C
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Expression in upper portion of the mammary sprout.
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Specimen
1F:
embryonic day 18.5
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
1F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
|
embryonic day 18.5
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
Regionally restricted |
1F
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Expressed in a luminal-like location.
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Specimen
1G:
embryonic day 18.5
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
1G:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
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Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1G
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Expressed in a luminal-like location.
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Specimen
2A/B:
postnatal day 14
(more )
Specimen
2A/B:
(close )
Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 14
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland |
Present |
Regionally restricted |
2A/B
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Mostly in the inner core of the ductal termini.
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Specimen
2C:
postnatal day 14
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
2C:
(close )
Genetic Background:
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involves: 129 * C57BL/6
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Age:
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postnatal day 14
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland duct |
Present |
Regionally restricted |
2C
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Mostly in the inner core of the ductal termini, and some co-stained for Krt14.
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Specimen
2D:
postnatal day 18
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
2D:
(close )
Genetic Background:
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involves: 129 * C57BL/6
|
Age:
|
postnatal day 18
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Sex:
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Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland |
Present |
Regionally restricted |
2D
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Expressed in inner cells of the ductal termini, and a few cells were double positive for Krt6 and Krt14.
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Specimen
2E:
postnatal week 3
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
2E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
|
postnatal week 3
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Sex:
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Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland duct |
Present |
Regionally restricted |
2E
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Expressed in terminal end buds, and in scattered ductal epithelial cells. Only a few cells co-stained for Krt14.
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Specimen
2F:
postnatal week 12
(more )
Note: Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
Specimen
2F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
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postnatal week 12
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Krt6; red - Krt14 (assay MGI:5827742).
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Specimen
not shown:
postnatal day 11
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
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postnatal day 11
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Sex:
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Female
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland |
Present |
Regionally restricted |
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Mostly in the inner core of the ductal termini, and some co-stained for Krt14.
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Specimen
not shown:
postnatal week 8
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
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Age:
|
postnatal week 8
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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