Gene Expression Data
Assay Details
Assay
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Reference:
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J:159506
Sun P, et al., Cytokeratin expression during mouse embryonic and early postnatal mammary gland development. Histochem Cell Biol. 2010 Feb;133(2):213-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5827742
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Gene symbol:
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Krt14
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Gene name:
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keratin 14
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Results
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Specimen
1A:
embryonic day 15.5
(more )
Note: Less developed mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Specimen
1A:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Less developed mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
|
Specimen
1B:
embryonic day 15.5
(more )
Note: More advanced mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Specimen
1B:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
More advanced mammary buds. Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
|
Specimen
1C:
embryonic day 16.5
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
1C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Specimen
1D:
embryonic day 18.5
(more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Specimen
1D:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1D
|
Mostly occupying the outer layers (Krt8-).
|
Specimen
1E:
embryonic day 18.5
(more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Specimen
1E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1E
|
Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
|
Specimen
1F:
embryonic day 18.5
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
1F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1F
|
Mostly occupying the outer layers (Krt6-). Many were also in the inner layer.
|
Specimen
1G:
embryonic day 18.5
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
1G:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1G
|
Mostly occupying the outer layers (Krt6-). Many were also in the inner layer.
|
Specimen
1H:
embryonic day 18.5
(more )
Note: Double labeled: red - Krt14; green - Krt8 (assay MGI:5827740).
Specimen
1H:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt8 (assay MGI:5827740).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1H
|
Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
|
Specimen
1I:
embryonic day 18.5
(more )
Note: Double labeled: red - Krt14; green - Krt19 (assay MGI:5827746).
Specimen
1I:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt19 (assay MGI:5827746).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland |
Present |
|
1I
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Three populations were seen, including Krt14+Krt19+, Krt14+Krt19-, Krt14-Krt19+.
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Specimen
1J:
postnatal day 0
(more )
Note: Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
Specimen
1J:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 0
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Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Krt14; blue - Krt6 (assay MGI:5827731); green - Krt8 (assay MGI:5827740).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: mammary gland |
Present |
|
1J
|
Mostly occupying the outer layers (Krt8-). Many were also in the inner layer and some of them co-expressed Krt8.
|
Specimen
2C:
postnatal day 14
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
2C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 14
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Specimen
2D:
postnatal day 18
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
2D:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 18
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Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Specimen
2E:
postnatal week 3
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
2E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal week 3
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Sex:
|
Female
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Specimen
2F:
postnatal week 12
(more )
Note: Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
Specimen
2F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal week 12
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt6 (assay MGI:5827739).
|
Specimen
3A:
embryonic day 15.5
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3A:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mammary gland primordium epithelium |
Present |
Widespread |
3A
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Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
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Specimen
3B:
embryonic day 18.5
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3B:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mammary gland epithelium |
Present |
Widespread |
3B
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Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
|
Specimen
3C:
postnatal day 11
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3C:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 11
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland epithelium |
Present |
Widespread |
3C
|
Nearly all epithelial cells expressed both Krt5 and Krt14, with some cells displaying stronger Krt14 while others stronger Krt5 signals.
|
Specimen
3D:
postnatal day 14
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3D:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 14
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Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland epithelium |
Present |
Widespread |
3D
|
The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
|
Specimen
3E:
postnatal day 18
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3E:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 18
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland epithelium |
Present |
Widespread |
3E
|
The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
|
Specimen
3F:
postnatal week 3
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3F:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal week 3
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland epithelium |
Present |
Widespread |
3F
|
The outer basal/myoepithelial cells expressed both Krt5 and Krt14, whereas some suprabasal/luminal cells expressed Krt14 but not Krt5.
|
Specimen
3G:
postnatal week 8
(more )
Note: Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
Specimen
3G:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal week 8
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Krt14; green - Krt5 (assay MGI:5827757).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mammary gland epithelium |
Present |
Regionally restricted |
3G
|
Expressed in the basal compartment. The number of suprabasal/luminal Krt14+ cells dramatically decreased, few such cells were found.
|
Specimen
not shown:
postnatal day 11
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6
|
Age:
|
postnatal day 11
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|