Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76826
Zuniga A, et al., Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis. Mech Dev. 2002 Jun;114(1-2):51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5881816
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Gene symbol:
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Hoxd13
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Gene name:
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homeobox D13
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Probe:
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probe A
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1J:
embryonic day 10.5
(more  )
Note: 32-35 somites
Specimen
1J:
(close  )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-35 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
1J
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Expression is posteriorly restricted.
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Specimen
1K:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
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Mutant Allele(s):
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Twist1tm1Bhr/Twist1tm1Bhr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-35 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Present |
Regionally restricted |
1K
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Authors state expression in mutant hindlimb bud remains simlar to wild-type.
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Specimen
1L:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
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Mutant Allele(s):
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Twist1tm1Bhr/Twist1tm1Bhr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-35 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Weak |
Regionally restricted |
1L
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Authors report that expression is downregulated and posterior restriction is lost in mutant forelimb bud compared to wild-type.
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Analysis Tools