Gene Expression Data
Assay Details
Assay
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Reference:
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J:85925
Moreau-Fauvarque C, et al., The transmembrane semaphorin Sema4D/CD100, an inhibitor of axonal growth, is expressed on oligodendrocytes and upregulated after CNS lesion. J Neurosci. 2003 Oct 8;23(27):9229-39
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5882750
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Gene symbol:
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Sema4d
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Gene name:
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sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
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Probe:
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Sema4d cDNA3
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1D:
postnatal day 10
(more )
Note: Double labeled: red - Sema4d; blue - Pdgfra (assay MGI:5882749).
Specimen
1D:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sema4d; blue - Pdgfra (assay MGI:5882749).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
1D
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Expression was detected in oligodendrocytes in the cerebellum. No more than 5% of the labeled oligodendrocytes coexpressed Pdgfra, which is mainly expressed at early stages of oligodendrocyte development.
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Specimen
1E:
postnatal day 10
(more )
Note: Double labeled: red - Sema4d; blue - Olig1 (assay MGI:5882751).
Specimen
1E:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sema4d; blue - Olig1 (assay MGI:5882751).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
Present |
Regionally restricted |
1E
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A high level of expression was noted. 95% of the labeled cells also expressed Olig1.
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Specimen
1F:
postnatal day 10
(more )
Note: Double labeled: red - Sema4d; blue - Olig2 (assay MGI:5882752).
Specimen
1F:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sema4d; blue - Olig2 (assay MGI:5882752).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
Present |
Regionally restricted |
1F
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A high level of expression was noted. 95% of the labeled cells also expressed Olig2, which represented ~20% of the Olig2-positive population.
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